Concordance between otic cytology and culture in diagnosis of external otitis canine by Malassezia spp
Journal Title: Revista MVZ Cordoba - Year 2015, Vol 20, Issue 3
Abstract
Objective. To determine the correlation between microbiological culture and otic cytology for diagnoses of external otitis by Malassezia in dogs. Materials and methods. 158 ear swabs of dogs with clinical diagnosis of external otitis were analyzed by cytology, mycological culture and metabolic tests. Results. Were obtained a positive results by cytology of 62% and 75.3% by culture. The 31.1% of isolates were identified as M. pachydermatis, 12.6% as M. furfur and 56.3% were classified as Malassezia spp., because was not possible to define the species. We found a positive concordance between cytology and culture for Malassezia spp., of 0.76 with a kappa index of 0.448 (95% CI 0.30 to 0.60) which represents a moderate strength of concordance between the two techniques, without regard the identified species. Conclusions. The use of a diagnostic test is not enough to establish the participation of Malassezia spp., as a causal disease agent.
Authors and Affiliations
Adriana Pulido-Villamarín, M. Sc *| Pontificia Universidad Javeriana, Facultad de Ciencias, Departamento de Microbiología, Unidad de Investigaciones Agropecuarias -UNIDIA-. Carrera 7 No. 43-82. Bogotá- Colombia.Correspondence: adriana.pulido@javeriana.edu.co, Rubiela Castañeda-Salazar, M. Sc| Pontificia Universidad Javeriana, Facultad de Ciencias, Departamento de Microbiología, Unidad de Investigaciones Agropecuarias -UNIDIA-. Carrera 7 No. 43-82. Bogotá- Colombia., Melva Linares-Linares, M. Sc| Pontificia Universidad Javeriana, Facultad de Ciencias, Departamento de Microbiología, Unidad de Investigaciones Agropecuarias -UNIDIA-. Carrera 7 No. 43-82. Bogotá- Colombia., Marcela Mercado-Reyes, M. Sc| Instituto Nacional de Salud, Dirección de Vigilancia y Análisis de Riesgo en Salud Pública, Subdirección de Prevención y Análisis de Riesgo. Avenida Calle 26 #51-20. Bogotá- Colombia.
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