CONDITION OF EYRTROCYTES AND HEMATOCRIT INDEX OF CANINE PRBCAT DIFFERENT STORAGE TERMS
Journal Title: Ukrainian Journal of Veterinary Sciences - Year 2018, Vol 1, Issue 285
Abstract
Unlike humane medicine, veterinary medicine does not have a unitary protocol on the duration of storage of packed red blood cells (pRBC) and its quality indicators. The aim of the work was to study the state of erythrocytes and the index of hematocrit in the canine pRBC during storage to determine the maximum period of its transfusion ability. For the study, 15 samples of the canine pRBC with the storage period of 1, 7, 14, 21, 28 and 35 days were used. The control points were the morphological parameters of erythrocytes, namely the change in their shape, as well as the hematocrit index. According to the results of the research, the least significant changes in the hematocrit index occur in the first three weeks of storage of canine pRBC. After 21 days of storage of erythrocytes, significant morphological changes are observed characterized by the appearance of echinocytes and spherocytes, and changes in the hematocrit index, namely, its increase from 65 ± 1.21 % for the first day to 75.8 ± 1.00% on the 35th day of its storage. Morphological changes of erythrocytes after 35 days of storage of canine pRBC do not allow to recommend it for transfusion to recipients. In the future, it is necessary to carry out clinical trials in vivo to determine the safety and efficacy of transfusion to recipients of pRBC with different periods of its storage.
Authors and Affiliations
I. M. Yakymchuk, A. O. Makarin, O. M. Yakymchuk, M. O. Marynyuk
Keywords
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