Confirmation of Endocrine Cells in the Pancreas of Iguana iguana with Immunohistochemistry
Journal Title: Scholars Journal of Agriculture and Veterinary Sciences - Year 2017, Vol 4, Issue 6
Abstract
Pancreatic endocrine cells of Green iguana (Iguana iguana) could not be distinguished clearly with routine histology staining (hematoxylin and eosin stain). Immunohistochemical method was carried out to detect the presences of endocrine cells producing insulin, glucagon, somatostatin, and pancreatic polypeptide (PP) in the pancreas gland of Green iguana. Pancreas glands of three adult Green iguanas were fixed with Bouin’s solution for 24 hour, processed with paraffin method, cut in 5 µm thicknesses, and stained by immunohistochemistry. The Immunoreactive (IR) endocrine cells were reported descriptively. Insulin- and glucagon-IR cells were showed in group of cells (islets of Langerhans) or individually dispersed in the whole pancreatic organ. Glucagon-IR cells seem more predominant than insulin-IR cells in the islets of Langerhans. Individual insulin- and glucagon-IR cells could be seen between the exocrine cells, but not as many as those found in the islets. Somatostatin-IR cells could be found in the islets of Langerhans. The amount of somatostatin-IR cells in the islets of Langerhans was less than glucogon-IR cells and insulin-IR cells. Glucagon-IR cells were the most prevalent cells compared to insulin- and somatostatin-IR cells. There was no PP-IR cells detected in the islets, around the duct of pancreatic exocrine parts, and between acinar cells. There were three major pancreatic endocrine cell types in the pancreas of Green iguana, with the most estimated amount being glucagon-IR cells, then insulin-IR cells were medium and somatostatin-IR cells were the least of all.
Authors and Affiliations
Yosua Kristian Adi, Teguh Budipitojo
Keywords
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