Confirmation of Proteins Encoding Genes Important in Bacterial Wilt Resistance
Journal Title: Asian Journal of Advances in Agricultural Research - Year 2017, Vol 1, Issue 3
Abstract
Background and Aim: Bacterial wilt caused by Ralstonia solanacearum is one of the most important soil borne plant pathogenic bacteria globally imparts decrease in crop yield per year. Situation can be improved by exploring defense mechanism against bacterial wilt. Aim of study was to confirm the expression of some defense genes differentially expressed in bacterial wilt resistant in comparison to medium resistant varieties. Place and Duration of Study: Study was done in Department of Biochemistry and Molecular Biology, University of Gujrat Pakistan for one year. Study Design: RNA extraction followed by cDNA synthesis was done following salicylic acid (SA) (1 mM) application on Solanum lycopersicum var. Roma (medium resistant) and Riogrande (resistant variety) for three consecutive days. Expression analysis studies of iron ATP-binding cassette (ABC) transporters, ATP synthase and chaperonin was done using RT-PCR along with measurement of antioxidants enzyme activities. Results: In control Iron ABC transporter and ATP synthase were expressed in both varieties though chaperonin was expressed in Roma only. Iron ABC transporter was expressed in both varieties within 1st day; while chaperonin was expressed after two and three days of SA treatment in Roma and Riogrande respectively. ATP synthase was expressed after one and three days of SA treatment in Roma and Riogrande respectively. Peroxidase and catalase activity increased in both varieties after one and three days more prominently in Riogrande; with little decrease after two days of SA treatment. Conclusion: Differential expression of ATP synthase and chaperonin in response to SA in resistant and medium resistant varieties of tomato signifies importance of these genes in response to bacterial wilt in tomato.
Authors and Affiliations
Ghulam Abbas, Nadia Zeeshan, Sabaz Ali Khan, Muzna Zahur, Amber Afroz
Keywords
Related Articles
- EP ID EP337275
- DOI 10.9734/AJAAR/2017/34529
- Views 68
- Downloads 0
Response of Chickpea Varieties to Different Irrigation Regimes
Chickpea is an important pulse crop in Bangladesh. The study was conducted at BINA sub-station Magura, to evaluate the yield potential of new cultivars of chickpea under different irrigation regimes. The experimental des...
Assessment of Herbicides and Pesticides Residues in Gella Surface Water
Presence of pesticides residues in ponds and earth dam waters of Gella Town used by the community as a means of water source was assessed. Water samples were collected in triplicates and residual pesticides determined us...
Estimation of Genetic Parameters Controlling Inheritance of Maize Quantitative Traits under Different Plant Densities Using Line × Tester Analysis
Type of gene action, heritability and genetic advance from selection are prerequisites for starting a breeding program for developing plant density tolerant variety of maize. The objective of the present investigation wa...
The Distribution of N, P and K in Abandoned Rubber Estate
Aims: This study examined the distribution of N, P and K in 30 years abandoned rubber estates in terms of soil fertility. Study Design: Ten blocks were established by using grid systems. Place and Duration of Study: The...
Haematological Parameters, Serum Metabolites and Enzyme Activities of Broiler Chicken Fed with or without Phytase
Aim: The experiment was conducted to investigate the effect of phytase on haematological indices, serum metabolites and enzyme activities of broiler chickens fed diets with or without phytase supplementation. Study Desig...