Construction of a droplet digital PCR detection system for platelet HPA-3 and HPA-15 genotyping
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 1
Abstract
Objective To establish a highly sensitive detection method of platelet HPA-3 and HPA-15 genotyping by droplet digital PCR (ddPCR), and to explore the feasibility of applying it to the detection of human platelet antigen (HPA) compatibility in maternal peripheral blood fetal free DNA. Methods For SNP mutation sites of HPA-3 and HPA-15, specific primers and MGB probes were designed, and amplification conditions such as annealing temperature and primer concentration of ddPCR were optimized to establish the optimal reaction system and clarify the test procedures. The methodological performance of the assay was evaluated, including specificity, sensitivity, repeatability and stability. ddPCR was used to detect 67 clinical blood samples, and the allele typing results were compared with the gene sequencing results. The fetal free DNA HPA antigen of 52 maternal peripheral blood samples was detected. Results The ddPCR method for detecting platelet HPA-3 and HPA-15 showed good specificity of primers and probes. The optimal annealing temperatures for HPA-3 and HPA-15 were 61.6℃ and 60.2℃, respectively. The optimal concentrations of primers were 900 nM and 700 nM respectively. The final concentration of the probe was 250 nM. The quantitative detection range of copy number was 2 to 20 000 copies, with lower limit of detection of 0.1 copies/μL, and the linearity is good. In low copy number samples, the intra - and inter batch coefficient of variation (CV) of actual detection values for HPA-3 and HPA-15 were both lower than 5%. The detection results of HPA-3 and HPA-15 genotypes of 67 blood samples were consistent with the gene sequencing results, and its application in fetomaternal platelet HPA-3, HPA-15 genotype detection met expectations. Conclusion The HPA-3 and HPA-15 ddPCR detection system constructed in this study has high accuracy, good repeatability, stability and sensitivity, and can be applied to the establishment of platelet HPA-3 and HPA-15 genotype donor pool, gene matching and fetomaternal platelet compatibility detection.
Authors and Affiliations
Xiaojiao KONG, Hongmei WANG, Shengbao DUAN, Tiemei LIU
Keywords
Related Articles
- EP ID EP738734
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.01.001
- Views 4
- Downloads 0
Confidential unit exclusion in Guangzhou from 2009 to 2022
Objective To investigate the condition of confidential unit exclusion(CUE) in Guangzhou, so as to ensure blood safety. Methods The number of CUE donors, demographic characteristics of CUE donors, reasons for CUE, and res...
Secondary herpes zoster wound cured by allogeneic platelet-rich plasma: a case report
Objective To investigate the feasibility of allogeneic platelet-rich plasma (PRP) for the treatment of herpes zoster wounds secondary to systemic lupus erythematosus (SLE), especially large ulcer wounds. Methods The trea...
Removing the interference of daratumumab on transfusion compatibility testing and transfusion efficacy comparison
Objective To explore the feasibility of blood transfusion compatibility testing for multiple myeloma(MM) patients treated with anti-CD38 monoclonal antibody daratumumab (DARA) after DARA-Fab fragment blocking, and to eva...
Autologous platelet-rich plasma cured refractory osteomyelitis complicated with fracture: a case report
Objective To share the experience of autologous platelet-rich plasma(PRP) combined therapy in successful treatment of refractory osteomyelitis with fractures in children. Methods One case of refractory osteomyelitis with...
Hepatitis E virus prevalence among blood donors in Wuhan urban agglomeration
Objective To investigate the prevalence of Hepatitis E virus (HEV) among blood donors in Wuhan urban agglomeration, aimed at providing data support for the development of HEV screening strategies for blood donors. Method...