Construction of Indonesian-Strain Avian Flu Virus Seed Vaccine Using Low Pathogenic Hemagglutinin Gene and Neuraminidase Pr8 Gene through Reverse Genetics
Journal Title: Journal of Vaccines and Immunology - Year 2017, Vol 3, Issue 1
Abstract
Avian Influenza H5N1 has been spreading in Indonesia since 2003. The Avian influenza virus H5N1 actually infects various animals, including birds, mammalian and others. Since 2005, avian influenza virus has infected humans. According to data, there are 132 persons suspected of contracting this virus, and this was one of the highest cases of avian influenza H5N1 outbreak in the world. One of the means of protection against infection is vaccination. Until now there is no H5N1 influenza vaccine extant, because the virus has a high level of pathogenicity, which makes it difficult to obtain a high yield of seed vaccine. In this research, we constructed H5N1 virus with low pathogenic Hemagglutinin gene and neuraminidase gene from PR8 (H1N1) using reverse genetic method. The new virus then inoculated in embryonated chicken eggs. We tested the H5N1 virus reverse genetic using molecular methods such as PCR and sequencing; and we used EID50 and Intravenous Pathogenicity Index for the pathogenicity test. The new H5N1 virus reverse genetic was also tested in mice to observe the antibody’s titer and challenge.
Authors and Affiliations
Nidom Reviany Vibriaanita, Alamudi Muh Y, Sillehu Sahrir, Indrasari Setyarina, Suindarti Rahmalia D, Qurnianingsih Ema, Dachlan Yoes P, Syahrani Ahmad, Rachmawati Kadek, Santoso Kuncoro P
Keywords
Related Articles
- EP ID EP553761
- DOI 10.17352/jvi.000021
- Views 43
- Downloads 0
Prospects for the Development of a Dengue Vaccine
Dengue is a mosquito-borne viral disease which is currently an important and rapid growing health problem across the globe. Four closely related dengue serotypes cause the disease, which ranges from asymptomatic infectio...
IMS 4112 and VLP of HBV as Th1 Adjuvants for a Recombinant Protein of HIV-1
Background: Current thinking suggests that vaccination approaches against the HIV-1 should be directed to elicit a Th1 cell-mediated immunity, neutralizing antibodies and/or ADCC mediating antibody responses. Also, exper...
Introduction of Stereo Chemical Constraints into β -Amino Acid Residues
Over the last 20 years, a large body of work in the literature has focused on the folded structures formed by peptide sequences containing backbone homologated residues. Currently increasing interest in peptide based vac...
Efficacy of a Recombinant Genotype VII Vaccine against Challenge with Velogenic Newcastle Disease Virus
Background: Newcastle disease virus (NDV) genotype VII has become the dominant genotype in China. However, NDV genotype II was used to make current commercial NDV vaccines. The mismatch of genotypes between circulating a...
Mild Encephalitis/Encephalopathy with a Reversible Splenial Lesion Associated with Rhinovirus Infection
We report a 7-year-old patient with mild encephalopathy with a reversible splenial lesion (MERS) presenting with recurrent delirious behavior, hallucinations and seizures following common cold. Cranial MRI showed high si...