CONTAMINATION OF BIOAEROSOL IN THE CONFECTIONERY FACILITY WITH Enterobacteriaceae MEMBERS
Journal Title: Folia Pomeranae Universitatis Technologiae Stetinensis Agricultura Alimentaria Piscaria et Zootechnica - Year 2018, Vol 45, Issue 340
Abstract
Airborne microorganisms from the production environment may be one of the main factor which lead to contamination of finished product or lead to decreasing of food quality. Good manufacturing practice includes a monitoring of purity in the production environment. It is important to estimate the source of microbial contamination and the influence of microclimatic conditions on changes in the microbial population of the air. The aim of study was to assess the occurrence of Enterobacteriaceae in composition of bacteriological bioaerosol from technological halls during production. The study were conducted in the confectionary facility in two technological halls (H.1, H.2) during production. In each hall, where the different stages of production were ran and various number of workers were worked, was determined 7 sampling sites. The air samples were taken by volumetric method with using MAS-100 impactor. Petri dishes were incubated in the standard conditions for the aerobic bacteria (22°C, 72 h). Isolates were stained by Gram’s technique and the members of Enterobacteriaceae were diversified by analysis the biochemical properties. Two-way ANOVA tests were performed to analyse number of bacteria as a function of sampling site and production hall. The results were given as CFU ∙ 1 m–3 of air. Statistical analysis were performed in the R Studio. The number of workers had positive correlations with the concentrations of bacterial bioaerosols. Enterobacteriaceae dominated in the sectors with the higher number of workers i.e. in sector hand – sorting and packaging of finished products.
Authors and Affiliations
Magdalena KRĘCIDŁO, Teresa KRZYŚKO-ŁUPICKA
Keywords
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