Continuous production of Urocanic acid by immobilized Pseudomonas aeruginosa species
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2018, Vol 8, Issue 6
Abstract
Urocanic acid (UA), an intermediate of L-histidine catabolism is commonly used as sunscreening agent in cosmetics and medicines. In the present work, an effort has been made to isolate the urocanic acid producing strain (Pseudomonas aeruginosa) and optimized at varied culture conditions in submerged fermentation like pH, temperature, incubation period and substrate concentration. Effect of carbon source, nitrogen source and metal ions were also studied. The results revealed that maximum Urocanic acid production was attained at pH 7.5 and temperature 40 0C using 0.4 % of histidine after 96 h of incubation. Carbon source (Glucose 2%, 2.104 mg/ml), nitrogen source (yeast extract 0.5 %, 1.936 mg/ml and metal ions (Mg++ 0.1mM, 1.523 mg/ml) further enhances the Urocanic acid production. Immobilization of Pseudomonas aeruginosa in 1.5 % of calcium alginate beads increased the yield up to 2.245 mg/ml. Moreover, repetitive batch operation and storage stability study of immobilized cells revealed maximum UA production up to five batches of operation. When stored at 4 0C, the immobilized whole cells remained stable up to 4 weeks. Hence immobilized whole cells of Pseudomonas aeruginosa were beneficial for industrial urocanic acid production.
Authors and Affiliations
Ragini G. Bodade, Prita S . Borkar
Keywords
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