CONTRIBUTION TO THE IMPROVEMENT OF IN VITRO PROPAGATION BIOTECHNOLOGY OF SYRINGA VULGARIS CULTIVARS

Journal Title: Current Trends in Natural Sciences - Year 2012, Vol 1, Issue 2

Abstract

The valuable biological, ecological and landscaping features of liliac, the real interest among the plant lovers, the increased requirements for the container culture, the limits imposed by conventional breeding techniques, the reduced quantity of planting material towards the requirements were the premisses to develop a technology for a rapid propagation material production of some cultivars of Syringa vulgaris by in vitro culture. The explants were represented by meristems with 2-3 leaflets and uninodal fragments sampled in December and June. The disinfection time varied with the stage of vegetation. The explants had a good behaviour on the initiation and multiplication culture media. 'Charles Joly' obtained the best result, with a growing percentage after innoculation of 92%. 'Mme. Lemoine' obtained the best multiplication rate of 12.33 microshoots/explant. The rooting phase was done in vivo on perlite substrate. The obtained results, 91% for 'Mme. Lemoine', 90.85% for 'Charles Joly' and 88.1% for 'Sensation' respectively, allowed us to recommend the usage of in vivo rooting on perlite substrate, simultaneously with the acclimatization, shortening the rooting time of microcuttings and production costs. The researches were expanded also on the fortification breeding material. The substrate made of Danmuld peat determined significant shoots’ growth for all three studied cultivars. In two years ‘Mme. Lemoine’ reached 16.4cm in height, ‘Charles Jolly’ 11.65cm and ‘Sensation’ 19.5cm in height. The number of suckers was between 3.4-6.1 suckers/plant and wasn’t influenced by genotype and culture substrate.

Authors and Affiliations

Mihaela Ileana Oprea

Keywords