Control of Phenolic Compound Secretion and Effect of Growth Regulators for Organ Formation from Musa spp. cv. Kanthali Floral Bud Explants
Journal Title: American Journal of Biochemistry and Biotechnology - Year 2006, Vol 2, Issue 3
Abstract
The study was undertaken to examine banana cv. Kanthali floral bud apex as an alternative source material for in vitro propagation because huge number of explants die due to microbial contamination in case of shoot tip explants. Contamination free cultures were established by treating the floral bud explants with 0.1% HgCl2 for 6 min. This study found that inflorescence tissues of experimental plant was almost contamination free but was high in phenolic compounds. Phenolic compounds secretion was successfully stopped by pre-soaking them in an antioxidant solution of 0.125% potassium citrate: citrate. After antioxidant treatment the floral bud explants were cultured on MS medium supplemented with different concentrations and combinations of BA+Kn+IAA/IBA+15%CW. Compact, white/greenish white callus was formed in different amount at all concentrations after 3 weeks of culture. All were again subcultured at same medium and after another 30-35 days at 2.0 mg L 1 BA+2.0 mg L 1 Kn+2.0 mg L 1 IAA+15%CW some callus showed embryogenic structure.
Authors and Affiliations
Soubir Titov
Keywords
Related Articles
- EP ID EP101993
- DOI -
- Views 95
- Downloads 0
Composite Scaffolds for Bone Tissue Engineering
Biomaterial and scaffold development underpins the advancement of tissue engineering. Traditional scaffolds based on biodegradable polymers such as poly(lactic acid) and poly(lactic acid-co- glycolic acid) are weak and...
Fabrication of 3-D Porous Mg/Zn doped Tricalcium Phosphate Bone-Scaffolds via the Fused Deposition Modelling
Three dimensionally interconnected porous resorbable beta-tricalcium phosphate (-TCP) ceramic scaffolds were developed using the indirect fused deposition modelling process. -TCP was doped with Mg and Zn, separately,...
Nanocrystalline Powders of Alkaline-Earth Phosphates as Precursors for Bioceramics
Bone is a nanocomposite made of calcium phosphates and collagen. Collagen has a typical fibrous structure, with diameter ranging from 100 nm to 2 µm. It is suggested that calcium hydroxyapatite, Ca10(PO4)6(OH)2 in the...
Bioceramics for Tissue Engineering Applications – A Review
Three dimensional (3-D) scaffolds have been explored in an attempt to persuade the body to heal or repair tissues that do not do so spontaneously. Considerable advances in tissue engineering and regeneration have been...
Nanoceramic Matrices: Biomedical Applications
Natural bone consisted of calcium phosphate with nanometer-sized needle-like crystals of approximately 5-20 nm width by 60 nm length. Synthetic calcium phosphates and Bioglass are biocompatible and bioactive as they bo...