Control of Phenolic Compound Secretion and Effect of Growth Regulators for Organ Formation from Musa spp. cv. Kanthali Floral Bud Explants
Journal Title: American Journal of Biochemistry and Biotechnology - Year 2006, Vol 2, Issue 3
Abstract
The study was undertaken to examine banana cv. Kanthali floral bud apex as an alternative source material for in vitro propagation because huge number of explants die due to microbial contamination in case of shoot tip explants. Contamination free cultures were established by treating the floral bud explants with 0.1% HgCl2 for 6 min. This study found that inflorescence tissues of experimental plant was almost contamination free but was high in phenolic compounds. Phenolic compounds secretion was successfully stopped by pre-soaking them in an antioxidant solution of 0.125% potassium citrate: citrate. After antioxidant treatment the floral bud explants were cultured on MS medium supplemented with different concentrations and combinations of BA+Kn+IAA/IBA+15%CW. Compact, white/greenish white callus was formed in different amount at all concentrations after 3 weeks of culture. All were again subcultured at same medium and after another 30-35 days at 2.0 mg L 1 BA+2.0 mg L 1 Kn+2.0 mg L 1 IAA+15%CW some callus showed embryogenic structure.
Authors and Affiliations
Soubir Titov
Keywords
Related Articles
- EP ID EP101993
- DOI -
- Views 126
- Downloads 0
Mapping QTLs Linked to Physio-Morphological and Plant Production Traits under Mapping QTLs Linked to Physio-Morphological and Plant Production Traits under
Drought stress is a major constraint for rice (Oryza sativa L.) production and yield stability in rainfed ecosystems. Identifying genomic regions (QTLs) contributing in drought resistance will help to develop rice cult...
Effective Thermophilic Composting of Crop Residues for Inactivation of Tobacco Mosaic Virus
An effective thermophilic composting bioreactor, in which a homogenous distribution of temperature was maintained at 63-65°C by the addition of a bioavailable carbon and low mixing, was developed. The bioreactor operat...
Control of Phenolic Compound Secretion and Effect of Growth Regulators for Organ Formation from Musa spp. cv. Kanthali Floral Bud Explants
The study was undertaken to examine banana cv. Kanthali floral bud apex as an alternative source material for in vitro propagation because huge number of explants die due to microbial contamination in case of shoot tip...
Biochemical Effects of Recombinant Porcine Somatotropin on Pig Fetal Growth and Metabolism: A Review
Prenatal development is mainly dependent on a close interrelation between nutritional supply use and regulation by hormones and growth factors. Mechanisms during early embryonic development are sensitive to manipulatio...
The Biology of p21 Waf1/Cip1 - Review Paper
p21 WAF1/Cip1 belongs to the Cip/Kip family of cyclin kinase inhibitors (CKI) (p21 Waf1/Cip1 , p27 Kip1 , p57 Kip1 ). p21 Waf1/Cip1 was first described as a potent and universal inhibitor of cyclin-dependent kinases (C...