Copper toxicokinetics in rats under prolonged administration of metal nanocomposites (Ag, Cu, Fe, Mn dioxide) with food
Journal Title: Біологія тварин - Year 2015, Vol 17, Issue 1
Abstract
Experimental model of nanocomposite of metals (NcMe) contained nanoparticles of Argentum (31.5±0.9 nm), Copper (70.0±5.0 nm), Iron (100.0±10.0 nm) and manganese dioxide (50.0±3.0 nm) in aliquots ratio with the final concentration of 100 µg/sm3 for each metal. The concentration of the metals in ionic form in the solution of mixture of salts — AgNO3, CuSO4 • 5H2O, MnSO4 • 5H2O and FeSO4 • 7H2O, respectively was equal to 100 µg/sm3 by metal. The experiment was carried out on mature male rats (n=80) of Vistar line. Observation of animals has been carried out for 90 days. On the 15 th , 30 th , 60 th and 90 th day of feeding the experimental sample NcMe and mixtures of salts of the metals from 5 animals in each group after euthanasia (provided by inhalation anesthetic chloroform) there were sampled organs and tissues, in which there was determined the content of copper by X-ray analysis. By the study of copper toxicokinetics in rats under the conditions of chronic experiment there was determined that metal nanoparticles at introduction in the biotic dose (0.3 mg/kg body weight), have better bioavailability than nano particulate form. For copper nanoparticles in biotic dose functional cumulation is characteristic, whereas for the toxic dose (4.0 mg/kg body weight) — the material cumulation dominates. Excretion of copper, injected to rats in nano- and macro particulate forms in biotic dose stabilized before the end of the experiment, whereas administration of toxic doses resulted in a gradual increase in metal excretion by the kidneys and lungs. Copper accumulation was found in the skin with hair and the testes of experimental rats, which received NcMe.
Authors and Affiliations
A. T. Kutsan, A. L. Orobchenko, M. Ye. Romanko
Keywords
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