Correlation between hypo-osmotic swelling test (host) and other seminal characteristics of deccani ram semen
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2017, Vol 5, Issue 0
Abstract
Integrity of sperm plasmalemma plays a pivotal role in the process of fertilization. The present study was undertaken to investigate the plasma membrane integrity by Hypo-osmotic swelling test (HOST) of Deccani ram sperm during liquid storage of semen (5°C) and its inter-relation with other seminal characteristics (Acrosomal integrity, livability, motility, sperm abnormalities and methyl blue reduction test (MBRT). Semen was collected from 8 Deccani rams (18 ejaculates/ram) and diluted (1:4; 200millions/ml) with egg yolk citrate extender (EYC) after assessment of semen volume (ml), concentration (millions/ml), colour, consistency and mass activity (0-5 scale). Seminal characteristics were evaluated immediately after dilution, after 24 and 48 h of storage at 5°C. The Pearson’s (r) correlation test was used to calculate correlations between Hos-test and other sperm parameters (Motility, Livability, Acrosome integrity, abnormalities and MBRT). The findings of the present investigation showed highly significant positive correlation between HOS coiling and motility (P<0.01), sperm livability (P<0.01) and acrosome integrity (P<0.01) which indicate its efficiency as feasible screening method for evaluation of male fertility prior to Artificial insemination.
Authors and Affiliations
M. Rajashri, K Ramchandra Reddy, G. Aruna Kumari, N Nalini Kumari , Surabhi Kesharwani
Keywords
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- EP ID EP428053
- DOI 10.18006/2017.5(2).195.200
- Views 55
- Downloads 0
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