Correlation between the proteins and protein profile(s) of different regions of epididymis and their contents in goat buck
Journal Title: Animal Science Papers and Reports - Year 2011, Vol 29, Issue 1
Abstract
Total proteins and their profile(s) in three regions of epididymis, its fluid and sperm membrane extract (SME) were analysed with an objective to determine the changes in spermatozoa during epididymal maturation in goat buck. The protein content from caput to cauda increased nonsignificantly (P>0.05) while significantly (P<0.05) in tissue homogenate and spermatozoa. SDSPAGE analysis indicated the removal of >205, 205 and 95 kDa; 40 kDa proteins only in the caput and corpus spermatozoa, respectively. The proteins with molecular weight of 10, 18, 25, 35 and 20 kDa, detected only in cauda and corpus spermatozoa, respectively, seem to be associated in the maturation process of spermatozoa during epididymal transit. SDS-Page analysis of tissue and fluid indicated that the >205, 205, 195, 200, 45, 25, 18, 15 and 12 kDa proteins are structural as well as secretory proteins because of their presence both in the epididymal tissue and fluid. The nature of 100, 97, 75 and 70 kDa was found to be purely structural because of their presence in the tissue only, whereas 90, 35, 6.5 and 3.0 kDa proteins, mainly of secretory nature, due to their detection only in the fluid. Therefore, the presence of 35, 25, 18 kDa proteins both in the epididymal fluid and cauda sperm indicate that these proteins are associated with goat buck sperm maturation and fertility. Therefore, it can be concluded that the proteins of 35, 25, and 18 kDa, associated with goat sperm maturation are similar to the mentioned molecular markers in other species and can be worked out as fertility markers for goat buck semen.
Authors and Affiliations
Ranjna S. Cheema, Amrit K. Bansal, G. S. Bilaspuri, V. K. Gandotra
Keywords
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