Correlation between the quantitative intensity of HLA-Ⅰ gene and its antibody and the clinical transfusion effect of matching platelets
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 11
Abstract
Objective To explore the influence of anti-HLA-Ⅰ with different mean fluorescence intensity (MFI) on the efficacy of HLA-A and -B gene matching platelet transfusion, so as to provide scientific data for clinical platelet gene matching transfusion strategy. Methods A total of 81 PTR patients had applied for HLA-Ⅰgene matched platelets from the platelet gene database established by our laboratory, and 28 (MFI <5 000) of them needed further avoiding of partial donor-specific antibodies and they were enrolled as the research subjects. According to the platelet MFI value of HLA-Ⅰ antibody-targeting antigen, they were divided into negative transfusion group (MFI <500) (group A) and positive transfusion groups (MFI≥500) ; the latter were further divided into group B (500≤MFI <1 000), group C (1 000≤MFI <3 000) and group D (MFI≥3 000) according to MFI value. Corrected count increment (CCI) in platelet count was used to compare the platelet transfusion effect in 4 groups. Results Among 28 platelet recipients with MFI <5 000, 19(67.86%) patients successfully received 72 effective transfusions. The first CCI (×109/L) in groups A, B, C and D were 10.27±7.46, 7.58±4.75 (P>0.05), 17.36±7.63 (P>0.05) and -0.77±2.30 (P<0.05), respectively. There was no statistical difference among group A, B and C. Conclusion The application of HLA-Ⅰ gene matching platelets in PTR patients can adjust the MFI threshold(<2 000) appropriately according to the patient′s condition without compromising the platelet transfusion effect.
Authors and Affiliations
Xinyu HUANG, Shu CHEN, Ying LIU, Bing ZHANG, Ji HE, Xianguo XU, Faming ZHU
Keywords
Related Articles
- EP ID EP738397
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.11.002
- Views 31
- Downloads 0
Effect of AB platelet secondary compatible transfusion on the efficacy of matched platelet transfusion
Objective To retrospectively analyze the effect of AB platelet secondary compatible transfusion on the efficacy of matched platelet transfusion. Methods A total of 2 276 cases of platelet transfusion in our hospital were...
Perioperative PBM in liver transplant patients: establishment and implementation
Objective To establish a practical and feasible blood management (PBM) model for clinical liver transplant patients, so as to optimize the perioperative blood usage and improve patient prognosis and survival rate. Method...
Matrix effect on the determination of Potency in Recombinant Coagulation Factor Ⅷ for injection
Objective To investigate the matrix effect on the determination of potency in Recombinant Human Coagulation Factor Ⅷ for Injection (rFⅧ). Methods Two different detection matrices were used to establish two methods for de...
Diversity of IgG autoantibodies of 4 Chinese intravenous immunoglobulins
Objective To develop methods to display the IgG autoantibody repertoire of intravenous immunoglobulin (IVIG) products, analyze the different types of antibodies and study the diversity of IgG autoantibody in 4 IVIG prepa...
Analysis of Del phenotype and genotype in RhD-negative blood donors in Guangzhou area
Objective To investigate the distribution, phenotype and genotype of D-elute type(Del) in blood donors with RhD negative blood in Guangzhou, so as to understand the molecular biological background of DEL blood group in t...