Correlation between the quantitative intensity of HLA-Ⅰ gene and its antibody and the clinical transfusion effect of matching platelets
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 11
Abstract
Objective To explore the influence of anti-HLA-Ⅰ with different mean fluorescence intensity (MFI) on the efficacy of HLA-A and -B gene matching platelet transfusion, so as to provide scientific data for clinical platelet gene matching transfusion strategy. Methods A total of 81 PTR patients had applied for HLA-Ⅰgene matched platelets from the platelet gene database established by our laboratory, and 28 (MFI <5 000) of them needed further avoiding of partial donor-specific antibodies and they were enrolled as the research subjects. According to the platelet MFI value of HLA-Ⅰ antibody-targeting antigen, they were divided into negative transfusion group (MFI <500) (group A) and positive transfusion groups (MFI≥500) ; the latter were further divided into group B (500≤MFI <1 000), group C (1 000≤MFI <3 000) and group D (MFI≥3 000) according to MFI value. Corrected count increment (CCI) in platelet count was used to compare the platelet transfusion effect in 4 groups. Results Among 28 platelet recipients with MFI <5 000, 19(67.86%) patients successfully received 72 effective transfusions. The first CCI (×109/L) in groups A, B, C and D were 10.27±7.46, 7.58±4.75 (P>0.05), 17.36±7.63 (P>0.05) and -0.77±2.30 (P<0.05), respectively. There was no statistical difference among group A, B and C. Conclusion The application of HLA-Ⅰ gene matching platelets in PTR patients can adjust the MFI threshold(<2 000) appropriately according to the patient′s condition without compromising the platelet transfusion effect.
Authors and Affiliations
Xinyu HUANG, Shu CHEN, Ying LIU, Bing ZHANG, Ji HE, Xianguo XU, Faming ZHU
Keywords
Related Articles
- EP ID EP738397
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.11.002
- Views 29
- Downloads 0
Evaluation of CLIA plus NAT for detecting HBsAg single-ELISA-reactive samples of blood donors
Objective To study the detection performance of HBsAg single-ELISA-reactive samples of blood donors. Methods Two kinds of ELISA reagents from different manufacturers (named as reagents A and B) were used for HBsAg screen...
Influence of mixing parameters in STAR automatic sample processing instruments on the detection results of internal controls
Objective To explore the significance of blending internal controls by automatic sample processing instruments in the enzyme linked immunosorbent assay (ELISA). Methods The internal controls were vortexed and mixed befor...
Preliminary study on HUVEC permeability changes in FNAIT induced by CD36 antibody
Objective To explore the pathogenesis of fetal edema caused by CD36 antibody in fetal/neonatal alloimmune thrombocytopenia (FNAIT), and to provide reference for clinical prevention and treatment. Methods The established...
Informationization and development strategy in blood collection and supply institutions in Beijing
[Objective] To investigate and analyze the current status, needs and issues of information system construction in blood collection and supply institutions in Beijing, and to provide strategic suggestions for the further...
Application of blood/fluid warmer during plateletpheresis in winter and its nursing
Objective To study the safety, effectiveness and nursing of blood/fluid warmer during the process of plateletpheresis in winter. Methods The blood re-transfusion speed during plateletpheresis in winter and the time of bl...