Correlation between Two Parameters of Mice Behaviour in the Open Field Test - - - Korelacija između dva bihevioralna parametra kod miševa u ,,open field’’ testu: poređenje rezultata dobijenih direktnim posmatranjem i snimanjem kamerom
Journal Title: Acta Facultatis Medicae Naissensis - Year 2017, Vol 34, Issue 4
Abstract
The open field test is being used extensively for the determination of different aspects of animal behaviour for over seventy years. The correlation between different behavioural parameters obtained in this test, although previously studied, is still debatable. Thus, we aimed to analyze and correlate behaviour scores to estimate the importance of individual parameters in this type of experiment. The open field test was performed on male BALB/c mice treated with either saline (10 ml/kg) or diazepam (2 mg/kg), one hour before the experiment. The behaviour scores (number of squares crossed and rearings performed) obtained either by video recording or direct observation, during a five-minute experiment, were compared using a t-test and were tested for correlation. As expected, diazepam caused an increase in the number of squares crossed and rearings performed by the animals. The number of rearings was statistically different between the groups monitored in two different ways. The correlations between the behaviour scores obtained in the two modes of monitoring for all groups were moderate/strong positive ones. The correlation analyses revealed that the amount of information conveyed by a single behaviour parameter, either the number of squares crossed or the number of rearings, could be sufficient to estimate the animals’ motor activity in the open field test. Also, the results of this test could provide clues to very important piece of information in drug discovery, i.e. the general animal behaviour under the influence of CNS acting drugs in an experiment. - - - ,,Open field’’ test se intenzivno koristi preko sedamdeset godina za procenu različitih aspekata ponašanja laboratorijskih životinja. Iako postoje publikacije koje razmatraju korelaciju između različitih parametara ponašanja životinja dobijenih u ovom testu, njihovi rezultati su još uvek nejasni. Iz ovih razloga, za cilj ove studije je postavljena analiza i međusobna korelacija parametara ponašanja životinja da bi se utvrdio značaj pojedinačnih parametara koji se mogu pratiti u ovom testu. U ovom radu, u ,,open field’’ testu, korišćeni su miševi muškog pola, soja BALB/c, koji su tretirani fiziološkim rastvorom (rastvor NaCl, 10 ml/kg) ili dijazepamom (2 mg/kg) jedan sat pre početka eksperimenta. Bihevioralni skor (broj pređenih kvadrata i broj vertikalnih pokreta (rearings)) dobijen u testu, bilo snimanjem ili direktnim posmatranjem, tokom petominutnog trajanja testa, upoređivan je t-testom i ispitano je postojanje korelacije među njima. Kao što je očekivano, aplikacija dijazepama je izazvala povećanje broja kvadrata koje je životinja prešla i broja vertikalnih pokreta. Nađeno je da se broj vertikalnih pokreta statistički značajno razlikovao kada je ovaj parameter određivan direktnim posmatranjem, odnosno snimanjem. Utvrđene su umerene i jake korelacije između bihevioralnih parametara. Korelaciona analiza je pokazala da se isti podaci mogu dobiti i iz samo jednog od ova dva bihevioralna parametra, a koji se odnose na procenu motorne aktivnosti životinja u ,,open field” testu. Takođe, rezultati ovog testa daju važan uvid u generalno ponašanje životinja pod uticajem lekova koji deluju na CNS.
Authors and Affiliations
Nikola M. Stojanović, Pavle J. Randjelović, Niko S. Radulović
Keywords
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- EP ID EP348917
- DOI 10.1515/afmnai-2017-0032
- Views 97
- Downloads 0
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