Correlation of Plasma Neutrophil Elastase Activity and Endogenous Protease Inhibitor Levels with the Severity of Pre-eclampsia
Journal Title: Journal of Clinical and Diagnostic Research - Year 2017, Vol 11, Issue 3
Abstract
Introduction: Pre-eclampsia (PE) is a common maternal syndrome characterized by severe systemic inflammatory response including neutrophil activation leading to uncontrolled activity of elastase. The excessive activity of elastase would lead to destroyal of the integrity of endothelial cells and could exacerbate the pathophysiological symptoms in PE. Thus, assessment of NE activity and its control mechanisms would be of relevance in the determination of severity of PE. Aim: To correlate the activity of plasma NE and its endogenous inhibitors α1 -antitrypsin (α1 -AT) and α2 -macroglobulin (α2 -MG) with severity of PE. Materials and Methods: A comparative study was conducted between normotensive pregnant (n=50) and pre-eclamptic (n=50) women. Serum C-Reactive Protein (CRP) was estimated by rapid latex slide and uric acid by uricase method. Plasma elastase was estimated using succinyl tri- L-alanyl-p-nitroanilide as substrate. Plasma α1 -AT, α2 -MG and NE- α1 -AT complex were quantified by ELISA. ANOVA and Pearson’s correlation tests were used to analyze the data. The results were expressed as mean±SD and p-value <0.001 was considered statistically highly significant. Results: The activity of elastase was increased significantly in severe PE (0.62±0.08) in comparison to normal (0.35±0.10) and mild pre-eclamptic subjects (0.37±0.03). The values of α1 -AT were significantly less in mild (83.94±25.08) and severe PE (68.58+26.39) in comparison to normal (110.26±42.39). There was a significant rise in the levels of α2 -MG in severe PE. However, the complex estimation did not evince any significant changes. Conclusion: The results of the present study indicate a significantly elevated elastase activity, α2 -MG levels and decreased α1 -AT in severe PE patients. The correlation analyses of PE severity parameters with NE, α1 -AT and α2 -MG further support the roles of these molecules in the assessment of severity of PE.
Authors and Affiliations
Mamatha Kunder, AV Moideen Kutty, V Lakshmaiah, SR Sheela
Keywords
Related Articles
- EP ID EP333974
- DOI 10.7860/JCDR/2017/24181.9469
- Views 101
- Downloads 0
Burden of Proteinuria and Risk Factors of Chronic Kidney Disease among Adult Population in Urban Puducherry, India
Introduction: In the recent times, Chronic Kidney Diseases (CKDs) are emerging as a serious problem all over the world along with diabetes mellitus and hypertension. The presence of proteinuria is considered as an indica...
Autoimmune Hepatitis – Primary Biliary Cirrhosis Overlap Syndrome
Autoimmune Hepatitis (AIH) and Primary Biliary Cirrhosis (PBC) are important immune mediated liver diseases. They are usually differentiated based on clinical, biochemical, serological and histological parameters. The pr...
Treatment of Proximal Humerus Fractures using PHILOS Plate
Introduction: Treatment of proximal humerus fractures always holds a dilemma for the treating surgeon. Aim: To assess the functional outcome of proximal humerus fractures treated with Proximal Humerus Internal Locking Sy...
Comparative Evaluation of Shear Bond Strength of Resin Modified Glass Ionomer Cement and Biodentine as Dentin Substitutes to Bonded Silver Amalgam and Composite Resin
ABSTRACT Introduction: The bond strength between the restorative material and the tooth, as well as between the restorative material and the base, is an important factor and can determine the longevity of a dental treatm...
Molecular Strain Typing of Clinical Isolates, Trichophyton rubrum using Non Transcribed Spacer (NTS) Region as a Molecular Marker
Introduction: Dermatophytes are a group of fungi which infect keratinized tissues and causes superficial mycoses in humans and animals. The group comprises of three major genera, Trichophyton, Microsporum and Epidermophy...