CORRELATIONS OF DEOXYRIBONUCLEIC ACID METHYLATION, HISTONE ACETYLATION, AND MICRORNA‑320 WITH SORBITOL DEHYDROGENASE IN DIABETIC RETINOPATHY

Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 2

Abstract

Objective: Prolonged and persistent hyperglycemia in diabetes mellitus (DM) leads to a variety of vascular complications, including the retinal disorder of diabetic retinopathy (DR). The mechanism of fructose formation of sorbitol assisted by sorbitol dehydrogenase (SDH) causing the loss of pericytes in the blood vessel is affected by epigenetic work comprised of deoxyribonucleic acid (DNA) methylation, histone acetylation, and microRNA‑320. This study aimed to determine the correlation of DNA methylation, histone acetylation, and microRNA‑320 with SDH in DR. Methods: This case–control study was conducted at a tertiary general hospital from July 2014 to June 2016. Study subjects were type 2 DM patients with and without DR, over 40 years old, suffered from DM for > 10 years. DNA methylation, histone acetylation, and microRNA‑320 were examined by real‑time quantitative polymerase chain reaction, while SDH level examination was carried out by enzyme‑linked immunosorbent assay. Analyses were performed with independent t‑test, Mann–Whitney, Spearman correlation, and multiple linear regression. Results: With respect to SDH, DNA methylation showed no significant correlation so as histone acetylation, in contrary to microRNA‑320 with a very strong negative correlation (r=−0.968, P < 0.005). Conclusion: MicroRNA‑320 was correlated to SDH in a manner of protective properties against the occurrence of DR. Involvement of DNA methylation and histone acetylation was perceptible in influencing SDH enzyme despite their insignificance if they took place individually.

Authors and Affiliations

RAMZI AMIN, DANY HILMANTO, ARIEF S. KARTASASMITA, HIKMAT PERMANA

Keywords

Related Articles

EVALUATION OF BIOACTIVE COMPOUNDS, ANTIOXIDANT, AND ANTIBACTERIAL PROPERTIES OF MEDICINAL PLANTS SAUROPUS ANDROGYNUS L. AND ERYTHRINA VARIEGATA L.

Objectives: The aim of the present study was to evaluate the bioactive, antioxidant, and antibacterial activity of methanolic extracts of medicinal plants Erythrina variegata L., and Sauropus androgynus L.Methods: Total...

INCIDENTAL DETECTION OF CARCINOMA IN SITU IN FIBROADENOMA OF BREAST IN A YOUNG WOMAN: A RARE FINDING

Fibroadenoma is the most common benign tumor of the breast in young females. Breast cancer arising within a fibroadenoma is a rare phenomenon. The incidence of carcinoma within a fibroadenoma is reported to be between 0....

THE ROLE OF THE CLINICAL AND MOLECULAR ASSAYS IN PROSTATE CANCER DETECTION

To assess the correlation between the clinical and molecular assays in identify early and robust prostate cancer detection. Early detection, management of cancer and decision about the disease are important for beneficia...

PATIENTS’ KNOWLEDGE, ATTITUDES, AND PRACTICES ON UNCOMPLICATED MALARIA MANAGEMENT IN PLATEAU STATE, NORTH-CENTRAL NIGERIA

Objectives: This study aimed to assess patients’ knowledge, attitudes, and practices (KAP) on uncomplicated malaria management in primary health-care (PHC) facilities of Plateau state, Nigeria. Methods: A validated self...

CHEMICAL AND BIOLOGICAL STUDY OF EUGENIA STIPITATA MC VAUGH COLLECTED IN THE COLOMBIAN ANDEAN REGION

Objective: The main objective of this study is to evaluate the chemical and bioactive properties of Eugenia stipitata (Myrtaceae) fruit seeds collected in the Andean region of Colombia using an ethanolic extract, a dichl...

Download PDF file
  • EP ID EP608814
  • DOI -
  • Views 101
  • Downloads 0

How To Cite

RAMZI AMIN, DANY HILMANTO, ARIEF S. KARTASASMITA, HIKMAT PERMANA (2019). CORRELATIONS OF DEOXYRIBONUCLEIC ACID METHYLATION, HISTONE ACETYLATION, AND MICRORNA‑320 WITH SORBITOL DEHYDROGENASE IN DIABETIC RETINOPATHY. Asian Journal of Pharamceutical and Clinical Research, 12(2), 475-478. https://europub.co.uk/articles/-A-608814