CovS modulates the antimicrobial susceptibility of Streptococcus pyogenes
Journal Title: Scientific Journal of Microbiology - Year 2014, Vol 3, Issue 4
Abstract
In Streptococcus pyogenes, the relationship between the major virulent regulatory factors, CovR/S, Mga, and Rgg, and antimicrobial susceptibility has not been elucidated till date. This study aimed to determine whether the inactivation of each regulatory factor affects antimicrobial susceptibility. We used covS, mga, and rgg knockout mutants from 3 different emm1 Streptococcus pyogenes strains with respect to the CovS amino acid sequence and investigated their susceptibility pattern to 29 antibiotics. Further, we investigated antimicrobial susceptibility of other 8 emm1 clinical isolates. The antibiotic susceptibility, particularly the susceptibility to penicillins, of the covS mutant strains was greater than that of the wild-type strains and the mga and rgg mutants. The covS complemented strain almost restored the susceptibility. The susceptibility to beta lactam in 1 strain with 2 amino acid sequence differences in CovS was higher than that of the other 2 strains with none or 1 amino acid sequence difference. Eight clinical isolates were classified into 2 groups based on antimicrobial susceptibility which correlate CovS amino acid sequence. The two-component signal transduction sensor protein CovS affects the susceptibility to antibiotics in Streptococcus pyogenes via the amino acid difference of CovS sequence.
Authors and Affiliations
M. Minami*| Department of Bacteriology, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, 467-8601, Japan., S. Torii| Department of Bacteriology, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, 467-8601, Japan., M. Ohta| Department of Nursing, Sugiyama Jyogakuen University, 17-3 Motomachi, Hoshigaoka, Chikusa-ku, Nagoya 464-8662, Japan.
Keywords
Related Articles
- EP ID EP1186
- DOI 10.14196/sjm.v3i4.1287
- Views 484
- Downloads 23
The antimicrobial activities of Ethanolic extracts of Basella alba on selected microorganisms
The antimicrobial effects of ethanolic extract of Basella alba against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albican was determined using the agar cup plate method. The phytochemica...
Factors influencing litter traits and body weight at pre-weaning ages among temperate rabbit breeds in the tropical conditions of Nigeria
The presentinvestigation was aimed at preliminary investigation of growth rate andprolificacy among temperate rabbit breeds namely, New Zealand White andCalifornia rabbits, in Kaduna State. Breed effect on litter traits...
Distribution of Aspergillus species among apparently healthy birds in poultry farms in Kaduna state, Nigeria
The study wasconducted to determine the prevalence and distribution of Aspergilllus species among apparently healthy birds in poultryfarms. Trachea swabs from 1500 birds in 52 commercial (10% of birds in eachpoultry farm...
Molecular detection of inv A and spv virulence genes in Salmonella typhimurium isolated from human and animals in Iran
In the present study, multiplex Polymerase Chain Reaction (m-PCR), simple PCR assay was used to confirm Salmonella typhimurium and detect Salmonella plasmid virulence (spvA, spvB, spvC) genes in human, bovine and poultry...
Comparison of capillary and venous blood using blood film microscopy in the detection of malaria parasites: A hospital based study
Malaria mainlydue to Plasmodium falciparum isassociated with great morbidity and mortality in Sub-Saharan Africa especiallyin children under 5 years. In order to eradicate the disease and avoidcomplications that may aris...