Creation of gene organisms in starting and ending regions of SicA gene of Salmonella enteritidis in pGEM vector
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2015, Vol 6, Issue 1
Abstract
Salmonella enteritidis is the most common cause of gastroenteritis. Sic A and Inv F activates transcription of sopB / sigD and sopE which coding effectively secreted proteins that enhance the code to be offensive. In this study upstream and downstream regions SicA gene of Salmonella enteritidis in pGEM vector was performed. In this study, the upstream and downstream genes SicA Salmonella enteritidis amplified based on specific primers and PCR and The fragment DNA cloned by T/A coloning method in pGEM vector and this constarct transformed into E. coli. The upstream and downstream SicA genes of Salmonella enteritidis was confirmed using PCR. The result of next steps showed that these regions were successfully cloned in E. coli. Confirmation of this conestract was done by digestion of restriction enzyme. According to the results ,can produce the conectruction via the recombination homologous and insertion of anti- biotic resistant genes between the upstream and downstream regions of SicA gene of Salmonella eneritidis as a candidate for gene vaccina against Salmonella eneritidis in future studies. Present study indicates the successful cloning of starting and ending encoder regions of SicA gene of Salmonella enteritidis in E. Coli bacteria. Thus, it seems that the structure produced in this study can be used as a gene vaccine candidate against Salmonella in future studies.
Authors and Affiliations
Sara Ostovari, Mohammadreza Gholami, Mohammad Zolfaghari, Ghasem Rahimi, Morteza khani
Keywords
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