Cross-sectional study evaluating the patterns of queries received by a newly established drug and poison information center in a tertiary care hospital
Journal Title: National Journal of Physiology, Pharmacy and Pharmacology - Year 2018, Vol 8, Issue 6
Abstract
Background: In recent decades, Pseudomonas aeruginosa has emerged as a multidrug-resistant bacteria by acquiring intrinsic resistance to a number of antimicrobial agents by uses distinctive resistant mechanisms to all the available antibiotics, which include metallo-β-lactamases (MBL) production, extended spectrum β-lactamase production, Amp C production, decreased permeability, altered penicillin binding proteins and rarely, and over expression of efflux pumps. Aims and Objectives: The aim of the study was to know the presence of IMP gene among P. aeruginosa isolates and to determine the carbapenem resistance mechanisms in carbapenem-resistant isolates of P. aeruginosa collected from sputum, blood, urine, pus, in Chennai, India. Materials and Methods: A total of 20 of non-repetitive clinical isolates of P. aeruginosa were collected and processed for biochemical tests and confirmed. Antibiotic susceptibility testing was determined by Kirby Bauer disc diffusion method. P. aeruginosa isolates were detected for the presence of blaIMP gene by polymerase chain reaction analysis. Results: Of the 20 clinical isolates of P. aeruginosa, 9/20 (45%) isolates were from sputum, 5/20 (25%) from blood, 3/20 (15%) from urine, and 3/20 (15%) from pus. Only 2/20 (10%) isolates showed sensitivity to imipenem. Other than that, for all other antibiotics isolates showed complete resistance 20/20 (100%). 17/20 (85%) clinical isolate of P. aeruginosa was found to possess blaIMP gene. Conclusion: The early detection of MBL‑producing P. aeruginosa may help in appropriate antimicrobial therapy and avoid the development and dissemination of these multidrug-resistant strains. However, to derive a conclusion, a more number of isolates are recommended and even other types of genes are also screened.
Authors and Affiliations
Varshan Raj, Gopinath Pa, Ashish R Jain
Keywords
Related Articles
- EP ID EP489611
- DOI 10.5455/njppp.2018.8.010039012018
- Views 55
- Downloads 0
Clinical profile of chronic daily headache in patient attending tertiary centre
Background: The frequent headache syndrome is among the most challenging disorder that conforms clinician on a routine basis. A major problem with the relatively new form chronic daily headache (CDH) has been its confusi...
Peripheral nerve conduction tests in patients with hypothyroidism and healthy volunteers: A comparative study
Background: The nervous system is the one of the most common sites get affected in patients with hypothyroidism and various tools have been developed for assessing it. Nerve conduction studies are one among them. Aims an...
Causality, severity and preventability assessment of adverse drug reactions in patients received anti-retroviral therapy in a tertiary care hospital: A retrospective study
Background: The cornerstone of management of patients with human immunodeficiency virus/acquired immunodeficiency syndrome (AIDS) infection is highly active antiretroviral therapy (HAART). However, antiretroviral drugs a...
A study of serum nitric oxide levels and pulse pressure in aging
Background: Nitric oxide (NO) is one of the major signaling messengers in the cardiovascular system. A sufficient level of endothelial NO is necessary to preserve normal vascular physiology and is one of the regulators o...
An in vitro study of antibacterial and antifungal activity of Cynodon dactylon
Background: Plant-derived antimicrobial agents remain an arena for research to overcome the issues pertaining to microbial resistance and adverse effects associated with synthetic drugs. Aims and Objectives: The present...