Cryopreservation of Dendrobium heterocarpum Lindl. via encapsulation-dehydration method.

Abstract

The species Dendrobium heterocarpum Lindl., a wild orchid native to north and northeast Thailand, creates an aura of mystique and grandeur with their intricate flower appearance which enthrals many collectors within the country. An increase in collection for trade or any orther purposes may lead to a dramatic decrase in the population of this species, thus becoming rare or endangered species in the near future. Cryopreservation is an alternative means for conservation of plant genetic resources which can be applied to this species. This study, therefore, was aimed to optimize the conditions for encapsulation-dehydration method in D. heterocarpum. The protocorms were successfully cryopreserved as the following protocol. The protocorms were encapsulated with Ca-alginate, precultured on solid ND medium supplemented with 0.25 M sucrose for 1 week in the dark at 0°C and subsequently precultured in liquid ND medium supplemented with 0.25 M sucrose for 2 days in the dark at 0°C. Then, they were dehydrated by air-drying for 12 hours and plunged into LN for 1 day. Recovery percentage was 8.33% after 2 weeks of regrowth. However, preculture with ABA was found to insufficiently enhance freezing and thawing tolerance. TTC staining was negative and further regrowth on solid ND medium confirmed no survival of the cryopreserved protocorms.

Authors and Affiliations

Witaya Pimda, Sumontip Bunnag

Keywords

Related Articles

Some brief considerations concerning the caves from Bistriţa-Năsăud county (Romania)

In Bistriţa-Năsăud county (Romania) there are several interesting caves, most of them being located in Rodnei Mountains. They are generally researched only for scientific issues, being closed for the public. In this pape...

Microbial production of poly-β-hydroxybutyrates -an overview

Efficient poly-β-hydroxybutyrate (PHB) production represents an actual preoccupation of fundamental research from current biotechnology. Since PHB production out of renewable sources has a positive environmental impact a...

The red neon, Paracheirodon axelrodi (Schultz, 1956), is able to survive in distilled water.

In the current paper we report on the ecophysiological adaptation of the red neon to extremely low water hardness (nearly zero). Such adaptations of aquatic vertebrate species (either fish or frogs) to extreme environmen...

Does natural selection explain the selforganization of the entire cosmos?

Because only enduring systems ultimately persist, physicist D. B. Kelley’s theory of universal selection, or “preservation of the stable,” expounds enormously upon the genesis of every lasting phenomenon in history, maki...

 Light environment and pigment composition of Megaceros pellucidus

The hornwort Megaceros pellucidus occupies wet, heavily shaded sites. In four sites in the central North Island of New Zealand the maximum photon flux density (PFD) was always less than 10 µmol m[sup]-2[/sup]s[sup]-1[/...

Download PDF file
  • EP ID EP107895
  • DOI -
  • Views 156
  • Downloads 0

How To Cite

Witaya Pimda, Sumontip Bunnag (2010). Cryopreservation of Dendrobium heterocarpum Lindl. via encapsulation-dehydration method. . Extreme Life, Biospeology & Astrobiology - International Journal of the Bioflux Society, 2(1), 7-14. https://europub.co.uk/articles/-A-107895