CU (II) ACCUMULATION BY MARINE NEUTROPHIL SULFUR-OXIDIZING BACTERIA
Journal Title: Мікробіологія і біотехнологія - Year 2019, Vol 0, Issue 1
Abstract
Aim: to determine the ability of neutrophilic sulfur-oxidizing bacteria to extract Cu (II) ions from aqueous solutions. Methods. The object of the study was the ability of marine sulfur-oxidizing bacteria isolated from the water of the Black Sea near the Odessa Bay to extract copper from aqueous solution. To study the metalaccumulating activity of sulfur-oxidizing bacteria, we have selected strains that were resistant to copper at the level of 0.01–0.02 mol/l. The initial concentration of Cu (II) in aqueous solution was 1 mM. The period of cultivation of microorganisms in aqueous solution containing copper was 10 days. The residual content of Cu (II) in aqueous solution was determined by the atomic absorption method on AAS-1 devices (Germany) and C-115PC Selmi (Ukraine) at the wavelength of 324.7 nm for Cu. The reliability of the results obtained was assessed on the Student's criterion with probability of p <0.05. Results. It was established that sulfur-oxidizing bacteria isolated from the water of the Black Sea capable of accumulating heavy metals from aqueous solutions, and Cu-accumulating activity depends on the duration of the interaction between microorganisms and solution. The maximum level of extraction of Cu (II) from aqueous solution was equal 89.24% was recorded on the 10th day of cultivation using a strain of Thiobacillus sp. DKZ_4. On the seventh day of cultivation, the extraction of Cu (II) from aqueous solution with using a strain of Thiobacillus sp. DKZ_2 reached 85.25%. It was shown that the level of resistance to copper, which had been previously defined, did not affect the ability of bacteria to accumulate copper. Conclusions. Neutral sulfur-oxidizing bacteria isolated from the water of the Black Sea are capable of extracting from aqueous solution from 22.83% to 89.24% of Cu (II) and therefore they can be used to develop biosorption biotechnology especially in the field of water purification from heavy metals. The ability to accumulate Cu depends on the strain, but does not depend on MIC.
Authors and Affiliations
N. Vasylieva, L. Sliusarenko, T. V. Vasylieva
Keywords
Related Articles
- EP ID EP670053
- DOI 10.18524/2307-4663.2019.1(45).164171
- Views 88
- Downloads 0
EFFECT OF LOW-INTENSITY LASER IRRADIATION ON THE CULTIVATED MACROMYCETES SEED CULTURE GROWTH ACTIVITY
Aim. The study of the effect of low-intensity laser radiation on the growth processes of spores and mycelia of cultured macromycetes. Methods. The research objects were pure cultures of basidiomycetes Flammulina velutipe...
AMOUNT OF ENDOPHYTIC MICROBIOTA IN GRAPEVINE SHOOTS
The aim of the investigation was to evaluate the amount of grapevine endophytic microbiota using different nutritional media. Materials and methods. Endophytic microbiota from wooden shoots of grapevine Vitis vinifera L....
STUDY OF CRYOINJURIES OF FREE AND IMMOBILIZED IN SODIUM ALGINATE GEL SACCHAROMYCES CEREVISIAE YEAST CELLS
The aim of this study was the revealing of the peculiarities of cryoinjuries of free and immobilized in sodium alginate gel Saccharomyces cerevisiae cells after cryopreservation. Methods. The research object was the Sacc...
BIOFILM FORMATION AND MOTILITY OF BACTERIA PSEUDOMONAS AERUGINOSA WITH DIFFERENT C-DI- GMPLEVEL
Aim: Biofilm formation abilities determination in Pseudomonasaeruginosa strains with different cyclic dimeric guanosine monophosphate (c-di-GMP) level and swimming, swarming, and twitching motilities. Materials and metho...
INFLUENCE OF NITRATE ON SULFIDOGENIC ACTIVITY OF BACTERIA DESULFOMICROBIUM SP. CRR3
The aim of this work was to study influence of nitrate on the effectivity of sulphate reduction and accumulation of hydrogen sulfide by bacteria Desulfomicrobium sp. CrR3. Methods. The object of the study was chromiumres...