CULTIVATION OF SUSPENDED CELL CULTURES AND BIOFILM OF ACIDITHIOBACILLUS FERROOXIDANS JCM 3863 IN PRESENCE OF COPPER IONS AND HIGH CONCENTRATIONS OF FERROUS IONS
Journal Title: Ecological Engineering and Environment Protection (EEEP) - Year 2018, Vol 1, Issue 1
Abstract
Bacteria Acidithiobacillus ferrooxidans are implemented usually in many biotechnological processes as bioleaching of metals from low grade ores and in some processes with ecological importance as purification of waste waters and gases, removal of metals from solid wastes. Suspended cultures and biofilm of Acidithiobacillus ferrooxidans JCM 3863 were cultivated in two media – with 15 and 18 g/l initial concentration of iron ions. Biofilm and suspended cultures have been cultivated in five consecutive cycles in each nutrient medium in presence of 5 g/l copper ions to complete oxidation of ferrous to ferric ions. In the end the biofilm thickness has been determined. Mean rates of oxidation were compared for each cycle of cultivation. In consecutive cultivation of suspended cells and biofilm in medium 15K after addition of copper ions the mean rates decrease in first three cycles, after that they increase in cycles 4 and 5. In consecutive cultivation of suspended cells in medium 18K the mean rates are different for all cycles. In biofilm in medium 18K after addition of copper ions there is decrease of mean rates compared to the first cycle, but they stay constant in the next cycles. Comparison of mean rates of ferrous ions oxidation shows that in biofilm they are higher than in suspended cultures. From results of presented research we can make the conclusion that the biofilm is better than suspended cells for industrial biotechnological processes in presence of copper ions and high concentrations of ferrous ions.
Authors and Affiliations
Kristina Lazarova, Vyara Mamatarkova
Keywords
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- EP ID EP383691
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