CULTURE ISOLATION OF MYCOBACTERIUM TUBERCULOSIS FROM SPUTUM SAMPLES USING MODIFIED LOWENSTEIN-JENSEN (LJ) MEDIA COMPARED WITH THE CONVENTIONAL LJ MEDIA
Journal Title: INTERNATIONAL JOURNAL OF MODERN PHARMACEUTICAL RESEARCH - Year 2018, Vol 2, Issue 4
Abstract
Tuberculosis (TB) is the most common cause of death due to a single infectious agent worldwide in adults. India alone accounts for 30% of the global tuberculosis burden. There is a need for a method of cultivation of mycobacteria that is reliable and economical and has a short turnaround time. The present study was attempted to assess the feasibility of using modified Lowenstein-Jensen (LJ) media, the isolation media for mycobacteria. They were compared with the conventional LJ medium, which was the gold standard. Sputum specimens from a total of 1812 clinically suspected cases of TB were studied. All the samples were decontaminated by using the modified Petroff’s method. Each sample was subjected to ZN staining and it was simultaneously inoculated onto the modified LJ medium and the conventional LJ medium. The growth from the cultures were confirmed by ZN staining and biochemical reactions. In DE-LJ the Mycobacterial growth was seen on the second week for +++ samples and on the third week for the ++ and + samples. . Mean number of weeks taken on three experimental slants was compared with mean number of weeks on control slants using using paired‘t’ test. The difference in mean number of weeks taken on supplemented medium was significantly less than that on unsupplemented medium. Modified Lowenstein-Jensen (LJ) media is a better medium as compared to the conventional LJ medium, both in terms of the number of isolates and the isolation rate. It was proved to be a very speedy method and it could isolate Mycobacterium tuberculosis 5- 10 days earlier as compared to the conventional L J medium.
Authors and Affiliations
Dr. Bernaitis L.
Keywords
Related Articles
- EP ID EP666592
- DOI -
- Views 202
- Downloads 0
PRESCRIPTION PATTERN OF PSYCHOTROPIC DRUGS IN SHAMBU GENERAL HOSPITAL, HORRO GUDURU WOLLEGA ZONE, SHAMBU TOWN, OROMIA REGIONAL STATE, ETHIOPIA
Back ground: Drugs are one of the health care component need to be used rationally and appropriate. The prescribing habits among psychiatrists can be improved by creating awareness about the choice of drugs according to...
A SYSTEMATIC REVIEW OF OREOSYCE AFRICANA HOOK.F. (FAMILYCUCURBITACEAE) PLANT PROFILE, BIOACTIVITIES AND CHEMICAL CONSTITUENTS
Attempts to wipe out the mosquito vector, which have been pursued for many years with synthetic insecticides is compromised by increased mosquito resistance to insecticides and use of inorganic insecticides raises enviro...
AFRICAN TRYPANOSOMIASIS: A REVIEW ON CHEMOTHERAPY
The increased drug development costs, the generally small drug budgets of developing countries and widespread evolution of drug resistance are primary causes. The most striking thing about that no new effective drugs for...
HEAVY METALS CONTAMINATION AND DETECTION BY USING INDUCTIVELY COUPLED PLASMA OPTICAL EMISSION SPECTROSCOPY: A REVIEW
Heavy metals are necessary for human health, and are beneficial when taken in to the body in foods or as supplements at appropriate, low levels but can be toxic when taken in higher concentrations. Heavy metals are conta...
CORRELATION AND PATH ANALYSIS FOR YIELD AND YIELD COMPONENT IN BLACK CUMIN (NIGELLA SATIVA L.)
Thirty-three accessions and three released varieties of black cumin genotypes were field evaluated at Debre Zeit Agricultural Research Center during 2015 main season using 6x6 simple lattice Design. The objectives were t...