Curcumin Effects on Hepatic Steatosis and Histopathology in an Obese Mouse Mode
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2015, Vol 5, Issue 8
Abstract
Aims: Curcumin is a popular spice and part of the ancient medicinal system Ayurveda. It is known to have anti-inflammatory, antimicrobial and antioxidant properties; research has shown curcumin to have beneficial effects on induced liver damage in animals. Study Design: Based on our own observations demonstrating hepatic improvement with curcumin in both obese and wild-type animal models, as well as the work of others, we determined that an n of at least 6 in each group at the selected time point would allow for greater than 80% probability of finding significant differences at a level of P< 0.05 between curcumin treated versus control fed OB-OB mice in the proposed experiment. Realizing that histomorphometric data are more variable and to ensure statistically valid data, the n per group was raised to 10, which allowed us to significantly detect a 10% difference between groups using a power analysis program and statistical evaluation of the data (Statistica, Statsoft). Place and Duration of Study: With a protocol approved by the University Animal Care Committee, we tested the hypothesis that the natural fatty livers in leptin k/o mice (OB/OB) would be improved by adding curcumin to food pellets at doses equal to human use (mg/ kg body weight). Methodology: Ten curcumin-treated and ten untreated control OB/OB mice were fed 2 months then studied for effects. Food pellets (Harlan-Teklad) were prepared with and without 180 mg curcumin in the daily dietary intake (9 gm). Calories for both diets: protein 15.2%, carbs 62.5%, fat 22.3%. Results: Histopathology on organs and cytokine analysis on serum parameters were measured on the two groups. Blood glucose was elevated in 8/10 curcumin-treated mice (p=0.07 a trend). Cytokines adiponectin and TNFα were strongly decreased by the curcumin diet and tissue superoxide was reduced. Liver % fat was significantly reduced by the curcumin additive, P=.01. Conclusion: Dietary curcumin in OB/OB mice produces significant improvement in liver health by reduction of % hepatic fat as well as by improved cytokine and inflammatory values. An increase in blood glucose by this treatment remains unexplained.
Authors and Affiliations
Asha Nookala, Betty Herndon, Agostino Molteni, Hamid Zia, Laura Alba, Jagdish Nachnani, Deepti Bulchandani
Keywords
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- EP ID EP347055
- DOI 10.9734/BJMMR/2015/13548
- Views 58
- Downloads 0
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