Current status of cutaneous leishmaniasis in some Iraqi Provinces: a cross-sectional study in 2015
Journal Title: Medical Science Pulse - Year 2018, Vol 12, Issue 1
Abstract
Background: Cutaneous leishmaniasis (CL) remains a serious public health concern in some parts of Iraq. This is the first study conducted to characterize Leishmania species causing CL among Iraqi patients using the sequence analysis of Internal Transcribed Spacer1 (ITS1). Aim of the study: To report of CL in some parts of Iraq, by different parasitological, cultural, and molecular methods. Material and methods: A total of 700 cases of suspected CL who were referred to the Iraqi’s clinics and health centers and they checked for Leishmania amastigote using Giemsa-stained, however the NovyMacneal Nicolle (NNN) culture led to the growth of promastigotes in all samples, then the samples were examined using PCR and RFLP-PCR methods. Results: The present study indicated that the prevalence of CL in eight Iraqi provinces as follow: Diwaniya 88 (15.1%), Wasit 84 (14.4%),Najaf 79 (13.6%), Thi-Qar 76 (13.1%), Basrah 67 (11.5%), Baghdad 65 (11.2%), Diyala 63 (10.8%) and Salah-Adin province which recorded the lower infection 60 (10.3%), and it appeared 83.3% by using Giemsa-smeared in eight Iraqi provinces. The highest infection (100%) appeared by using PCR while the lowest infection (68%) appeared by culture on NNN media. The present study was revealed that the highest infection (60%) caused by L.major than L.tropica (40%). Our results showed that 368 (52.6%) of CL patients were had single lesion and 215 (30.7%) had multiple lesions, and the ulcerative wet type lesions were present in 49.6%, while the nodule dry type lesions were present in 33.7%. Conclusions: The diagnosis of CL classically by Giemsa-smeared and in vitro cultivation required the presence of a relatively high number of viable or morphologically intact parasites; this may pose a problem particularly in the chronic phase of CL where parasite levels in skin lesions are very low. The PCR-based checking of Giemsastained smears appears to be reasonably, sensitive and specific in revealing the presence of Leishmania parasites in such chronic lesions.<br/><br/>
Authors and Affiliations
Magda A. Ali, Ali Khamesipour, Abdulsadah A. Rahi, Mehdi Mohebali, Amir Ahmad Akhavan, Alireza Firoozi
Keywords
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- EP ID EP274603
- DOI 10.5604/01.3001.0011.6451
- Views 148
- Downloads 0
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