Cytochemical identification of endocrine thymus of chicken in relation to aging
Journal Title: Veterinary Research Forum - Year 2013, Vol 4, Issue 3
Abstract
Age related cytochemical changes of thymic endocrine cells were studied in 78 day old chicks at five day interval to age of day 60 employing a panel of cytochemical stains. Methenamine silver revealed cell morphology including cell processes distinctly while diamine silver revealed a stronger argentaffinity in these cells. The cells had greater affinity for diamine silver compared to methenamine silver (Argentaffin), followed by formaldehyde induced auto-fluorescence, argyrophilia, lead hematoxylin and HCl-toluidine. The chromaffin reaction was the weakest. Cytochemically, three different endocrine cell populations i.e. argentaffin cells, argyrophilic cells and amine precursor uptake and decarboxylation series (APUD)/chromaffin cells, formed the resident population of thymic endocrine cells. Occurrence of numerous serotonin storing cells, moderately frequent APUD cells, and fewer chromaffin as well as mast cells suggests for a conspicuous reservoir of amine storing cells in thymus. Morphologically argentaffin cells were of four types i.e. the peripherally granulated spherical cells (Type-I), densely granulated oval cells (Type-II), pyramidal argentaffin cells (Type-III) and diffusely granulated elongated cells (Type-IV). The type-II argentaffin cells were most frequent in the medulla followed by the type-I cells and the type-III cells. The type-IV cells were least in frequency. The age related changes in frequency of these cells are also discussed.
Authors and Affiliations
Uma Mishra
Keywords
Related Articles
- EP ID EP104261
- DOI -
- Views 122
- Downloads 0
Modeling the growth of Staphylococcus aureus as affected by black zira (Bunium persicum) essential oil, temperature, pH and inoculum levels
Black zira (Bunium persicum) is a medicinal plant and spice, naturally grows in Iran. The aim of this study was to investigate the combined effects of different concentrations of Bunium persicum essential oil (EO; includ...
Effects of in vitro copper sulphate supplementation on the ejaculated sperm characteristics in water buffaloes (Bubalus bubalis)
This study was carried out to investigate effects of copper sulphate (CuSO4) additive to semen extenders on sperm parameters: progressive motility, viability, membrane integrity and DNA damage, after semen dilution and c...
Follicle stimulating hormone increases spermatogonial stem cell colonization during in vitro co-culture
The complex process of spermatogenesis is regulated by various factors. Studies on spermatogonial stem cells (SCCs) have provided very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of tot...
Designing and evaluation of Dot-ELISA for diagnosis of Fasciola infection in cattle
Fasciolosis is a disease caused by liver fluck of the genus of Fasciola. Diagnosis of fasciolosis has been challenging for a long period due to low sensitivity of the coprological diagnostic method. In this study, an in-...
Molecular and clinical study on prevalence of feline herpesvirus type 1 and calicivirus in correlation with feline leukemia and immunodeficiency viruses
Upper respiratory tract diseases (URTD) are common clinical problem in cats worldwide. Feline calicivirus (FCV) and feline herpesvirus type 1 (FHV-1) are the main primary pathogens. Feline immunodeficiency virus (FIV) an...