Cytological and microbiological evaluation of uterine washing of bovine with reproductive problems (preliminary study)
Journal Title: Revista MVZ Cordoba - Year 2011, Vol 16, Issue 3
Abstract
Objective. Determination of the cytology and microbiology characteristics using low volume uterine lavage in dairy cows with reproductive problems in two herds on the cundiboyacence plateau. Materials and methods. 21 uterine lavage samples from cows with reproductive problems in two dairy herds were evaluated. The herds were located in Sibate (Cundinamarca) and Ventaquemada (Boyaca). 10 and 11 cows were sampled in each herd respectively. The uterine lavages were examined both macroscopically and microscopically. The cytological evaluation was done using Gram and Wright staining. The determination of the bacterial flora was done using standard microbiological methods used for the identification of the bacteria, the Kirby-bauer diffusion test for sensitivity was also done. Results. Bacilli, cocci, desquamating uterine epithelial cells (CEDU) and inflammatory response (IR) mediated by polimorphonuclear cells were observed. Bacterial isolates were lactobacillus sp as part of normal floral (16.66%) and Klebsiella sp as accompanying flora (16.66%). There were also isolations of pathogenic bacteria that cause reproductive problems such as o Streptococcus sp. β hemolytic (33.33%), Streptococcus sp. α hemolytic (50%) y Streptococcus sp. γ hemolytic (50%) and Arcanobacterium pyogenes (Corynebacterium- Actinomyces pyogenes) (16.66%); spirochaete was observed in 9.52% of the samples. Conclusions. There was a direct relationship between macroscopic appearance of the uterine lavage, citology response and bacterial isolation.
Authors and Affiliations
Milena Sánchez L, Microb Agrí Vet| Pontificia Universidad Javeriana. Facultad de Ciencias. Departamento de Microbiología, Bogotá D.C. Colombia., Carolina González C, Microb Agrí Vet| Pontificia Universidad Javeriana. Facultad de Ciencias. Departamento de Microbiología, Bogotá D.C. Colombia., Rubiela Castañeda S, M. Sc *| Pontificia Universidad Javeriana. Facultad de Ciencias. Departamento de Microbiología, Bogotá D.C. Colombia.Correspondencia: castaneda.r@javeriana.edu.co, Adriana Pulido V, M. Sc| Pontificia Universidad Javeriana. Facultad de Ciencias. Departamento de Microbiología, Bogotá D.C. Colombia., Humberto Guáqueta M, Ph. D| Universidad Nacional de Colombia. FMVZ, Clínica de la Reproducción. Bogotá D.C. Colombia., Moises Aranda S, M. Sc| Pontificia Universidad Javeriana. Facultad de Ciencias. Departamento de matemáticas. Bogotá D.C. Colombia., Milton Rueda V, Ph. D| Pontificia Universidad Javeriana. Facultad de Ciencias. Departamento de matemáticas. Bogotá D.C. Colombia.
Keywords
Related Articles
- EP ID EP3221
- DOI -
- Views 302
- Downloads 17
NUTRITIONAL CHARACTERISTICS OF THE INDIAN ALMOND (Terminalia catappa L.) AS A SUPPLEMENT IN ANIMAL FEEDING
Objective. to evaluate the nutritional uses of Indian almond seed Terminalia catappa for three states of ripeness (green E1, intermediate E2 and E3 ripe) as a supplement alternative in animal feeding. Materials and metho...
The efficacy of chloroquine treatment against naturally occuring Giardia duodenalis infection in lambs
Objective. The aim of the present study was to determine the efficacy of Chloroquine (Cq), an antimalarial medicine, administered at a dose of 2.5 mg/kg, orally, during 5 consecutive days, in Sakiz and Merino lambs natur...
CHEMICAL COMPOSITION AND RUMINAL DEGRADABILITY OF TEN FODDER CULTIVARS OF Leucaena leucocephala
Objective. The chemical composition and ruminal degradability of ten cultivars of Leucaena leucocephala Lam de Wit were examined using principal components analysis (PCA). Materials and methods. Chemical composition, sec...
Coat genetic markers of the domestic cat Felis catus (Felidae) from southwestern Colombia
Objectives. Establish the genetic profiles of cats from 12 neighboring municipalities in southwestern Colombia, in a town course from Pereira-Popayán. Estimate the degree of diversity, genetic structure, and quantify gen...
IMPORTANCE OF THE USE OF DIFFERENT DIGESTIBILITY TECHNIQUES IN PIG NUTRITION AND FOOD FORMULATION
The nutritional value of a meal for pigs could be expressed using a digestibility coefficient whereas the type of digestibility may be assessed by the point of origin of the sample: 1) ileal Digestibility (IDI), attempts...