Cytological Patterns and Molecular Detection of Methicillin Resistance Staphylococcus Aureus in Buccal Mucosa among Local Tobacco Users
Journal Title: Advance Research Journal of Multidisciplinary Discoveries - Year 2019, Vol 33, Issue 33
Abstract
Objectives: This study was carried out to assess the cytological changes and to detect methicillin resistant staphylococcus aureus in oral smears among local tobacco users in Port Sudan city, Sudan, using Cytological Pap smear and PCR technique to help in detection of MRSA among local tobacco users. Materials and Method: One hundred buccal smears and oral swabs were obtained randomly from local tobacco users. The specimens were cytologically prepared and examined, swabs were cultured on Blood Agar with novobiocin disc and Mannitol Salt Agar for primary isolation of Pathogen.Then mecA gene detection was done used PCR technique. Result: 32% subjects were identified with keratinization, 21% of the users were identified with bacterial infection and moniliasis, inflammation was present in 18% of the users in the Pap smear. A 70 Staphylococcus aureus was isolated from the mouth swab, and 14 MRSA was isolated out of this 70 Staphylococcus aureus, 1 out of this were positive for mecA gene. Cytological changes and MRSA isolate was found to be high among Tobacco consumer for long period using rather than those who tobacco consuming for short period. The result revealed that the antimicrobial resistance of S. aureus for Methicillin was (20%). Conclusion: This study concluded that there is correlation between tobacco use and duration of use and increasing susceptibility to MRSA infection and cytological changes.
Authors and Affiliations
Manar G. Shalabi, Israa M Omer, Anass M Abbas
Keywords
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