Cytological Patterns and Molecular Detection of Methicillin Resistance Staphylococcus Aureus in Buccal Mucosa among Local Tobacco Users
Journal Title: Advance Research Journal of Multidisciplinary Discoveries - Year 2019, Vol 33, Issue 33
Abstract
Objectives: This study was carried out to assess the cytological changes and to detect methicillin resistant staphylococcus aureus in oral smears among local tobacco users in Port Sudan city, Sudan, using Cytological Pap smear and PCR technique to help in detection of MRSA among local tobacco users. Materials and Method: One hundred buccal smears and oral swabs were obtained randomly from local tobacco users. The specimens were cytologically prepared and examined, swabs were cultured on Blood Agar with novobiocin disc and Mannitol Salt Agar for primary isolation of Pathogen.Then mecA gene detection was done used PCR technique. Result: 32% subjects were identified with keratinization, 21% of the users were identified with bacterial infection and moniliasis, inflammation was present in 18% of the users in the Pap smear. A 70 Staphylococcus aureus was isolated from the mouth swab, and 14 MRSA was isolated out of this 70 Staphylococcus aureus, 1 out of this were positive for mecA gene. Cytological changes and MRSA isolate was found to be high among Tobacco consumer for long period using rather than those who tobacco consuming for short period. The result revealed that the antimicrobial resistance of S. aureus for Methicillin was (20%). Conclusion: This study concluded that there is correlation between tobacco use and duration of use and increasing susceptibility to MRSA infection and cytological changes.
Authors and Affiliations
Manar G. Shalabi, Israa M Omer, Anass M Abbas
Keywords
Related Articles
- EP ID EP461936
- DOI -
- Views 58
- Downloads 0
Phenotypic variability and characterization of the body conformation of Brazilian Sport Horse, Breton Postier and Brazilian Donkey equine breeds
This study aimed to characterize morphologically equines of the Brazilian Sport Horse (BSH), Breton Postier (BP) and Brazilian Donkey (BD) breeds, in order to discriminate morphological patterns within and between herds,...
The Effect of Personality Traits and Burnout Levels in Trinity School of Medicine Students
Overwhelming negative stress can lead to burnout in medical students, which can cause a decrease in academic performance. Several factors can play a role, such as personality, work/school environment, and home life. Pers...
Effect of body mass index on Cardiac output after Coronary Angioplasty in patients of Acute Myocardial Infarction
Background & Objective: Generally overweight and obese are at more risk of developing cardiovascular diseases. But few researchers found that when coronary artery disease (CAD) developed in overweight and obese patients,...
Physio-Chemical assessment of water sources for drinking purpose in Badin City, Sindh Province, Pakistan, (Water Supply Schemes and Hand Pumps)
Recently, water bodies contain several types of chemicals and the quantity is more than there were couples of years ago. Clean and safe drinking water is one of the basic needs of life and society. Pakistan is the countr...
The Macho Man: Untold Experiences
This study presents a social illness and considered one of the chronic problems among males in all walks of life; the male person delve on their lifestyle, experiences, causes, modes operandi and their transactions of th...