Cytoprotective Activity of Citrus aurantifolia fruits Extract Against Aflatoxin-B1 induced Cytotoxicity
Journal Title: International Journal of Research in Pharmacology & Pharmacotherapeutics (IJRPP) - Year 2013, Vol 2, Issue 2
Abstract
Citrus aurantifolia, is one of the herbal medicines in India that has been commonly used for treating various diseases, including liver disorders. Based on its use in traditional oriental medicine, the current study was carried out to evaluate the cytoprotective effects of C. aurantifolia using Aflatoxin B1 (AFB1)-induced liver injury in a rat model.Wistar albino rats were divided into five groups of six animals each. Group I served as the control. Group II was treated with vehicle, dimethyl sulfoxide (DMSO) a single intraperitoneally (i.p) on day 5. Group III animals received AFB1-alone (1mg/Kg body weight) intraperitoneally in DMSO as a single dose on day 5. Group IV and V received MeCA and AqCA (500mg/Kg body weight, per oral) for 5 days and AFB1 (1mg/kg body weight) intraperitoneally in DMSO as a single dose on day 5. At the end of the 8th day, the liver was collected and used to determine the hepatoprotective activity. Genomic DNA fragmentation was observed by agarose gel electrophoretic pattern in the rat livers. The ultra-structure of the liver cells was studied by electron microscopy. C. aurantifolia treatment significantly protected nucleic acid levels. We also found that the treatment significantly inhibited DNA fragmentation. Nucleus structures were well maintained. These results demonstrate that C. aurantifolia has a cytoprotective effect against AFB1-induced liver injury. In this study, we focused on the cytoprotective effects of C. aurantifolia against AFB1-induced cytotoxicity.
Authors and Affiliations
Prakash Srinivasan Timiri Shanmugam
Keywords
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