Cytoprotective activity of mulberry leaf extract against oxidative stress-induced cellular injury in rats
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2013, Vol 26, Issue 1
Abstract
Morus alba Linn. (MA), mulberry leaves have been used as a beverage for prevention of various diseases including hyperlipidemia and hyperglycemia. Recently, the antioxidant activities of the MA leaf extract have been reported. The objective of this study was to investigate the effect of the MA leaf extract on free radical-induced cellular injury. In the in vitro models, the extract scavenged stable free radical (1, 1-diphenyl-2-picrylhydrazyl; DPPH) in a concentration-dependent manner with an IC50 of 20.10±0.78 g/ml. The extract protected the erythrocytes from free radical (2, 2’-azobis (2-amidinopropane) dihydrochloride; AAPH)-induced hemolysis with an IC50 of 74.22±9.87 g/ml. Additionally, the extract significantly prevented the gastric mucosal injury induced by ischemia-reperfusion (I/R) in rats when given orally at doses of 0.25 and 0.50 g/kg/day for 3 consecutive days (p<0.05; n=7). However, this effect was not found when the higher doses (1 and 2 g/kg/day) of the extract were tested. In conclusion, these results indicate that the MA leaf extract possesses the cytoprotective activity against free radical-induced cell injury. Therefore, when given at the appropriate dose range, the mulberry leaf may potentially be used as a food supplement in patients with certain diseases in which the oxidative stress-induced cellular injury is pathologically involved.
Authors and Affiliations
Atika Jaruchotikamol, Patchareewan Pannangpetch
Keywords
Related Articles
- EP ID EP161450
- DOI -
- Views 91
- Downloads 0
Biological activities of Morus celtidifolia leaf extracts
The aims of this research were to examine the antibacterial, cytotoxic and antiradical/antioxidant activities of the organic extracts obtained from the leaves of the medicinal plant Morus celtidifolia (Family: Mora...
The clinical research for Ganoderan's effect on preventing and treating cerebral arteriosclerosis through inhibiting NADPH oxidizing enzyme expression
A lot of researches have verified that produced excessive reactive oxygen is one of the hazard factors causing atherosclerosis. NADPH oxidase is the main protease of vascular cell’s producing reactive oxygen, the e...
Analysis on pathogen distribution and drug-resistance of incision infection caused by vascular operation
To investigate pathogen distribution and drug resistance of incision infection caused by vascular operation to reduce postoperative incision infection, this paper retrospectively reviewed and analyzed 635 in-hospit...
Evaluation of nutritional and sub-acute toxicological study of plant based supplement of Achyranthes aspera
The present study was conducted for the nutritional, microbiological and toxicological evaluation of test compound having main ingredient Achyranthes aspera. Nutritional value assessment, microbiological analysis a...
Antioxidant study of flavonoid derivatives from the aerial parts of Rhus natalensis growing in Saudi Arabia
Phytochemical study of the CH2Cl2 soluble fraction of the aerial parts of R. natalensis resulted in the isolation and identification of six flavonoid derivatives, -amyrin and -sitosterol glucoside (daucosterol)....