Cytotoxic Activities of Certain Medicinal Plants on Different Cancer Cell Lines
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2017, Vol 14, Issue 3
Abstract
Objectives: In recent years, the use of plants for the prevention and treatment of cancer is gaining more attention due to their diverse range of phytochemical constituents and fewer adverse effects. In this study, four medicinal plant species from the Kars province of Turkey were investigated for their cytotoxic potential against six different cancer cell lines and one normal cell line. Materials and Methods: MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-dipenyltetrazolium bromide] assay was performed to assess cytotoxic activity and apoptotic effect was determined using flow cytometry and caspase-3 analyses. Results: Significant cytotoxicity (≥70%) was observed with the leaf extract of Artemisia absinthium on A-549, CCC-221, K-562, MCF-7, PC-3 cells, whereas seed extracts caused significant cytotoxicity (≥70%) on CCC-221, K-562, MCF-7, PC-3 cells. Selective cytotoxicity was obtained with leaf extract on A-549 and K-562 cells; and with seed extract on K-562, MCF-7 and PC-3 cells compared with normal Beas-2B cells. The levels of cytotoxicity for both extracts were time- and dose-dependent at lower concentrations. Moreover, selective cytotoxicity (78%) was detected on A-549 cells with the seed extract of Plantago major. Cytotoxicity of extracts from Hyoscyamus niger and Amaranthus retrosa ranged between 10% and 30%. Conclusion: A. absinthium extracts and P. major seed extract have potential for development as therapeutic agents for cytotoxicity on certain cancer cells following further investigation.
Authors and Affiliations
Deniz UĞUR, Hatice GÜNEŞ, Fatma GÜNEŞ, Ramazan MAMMADOV
Keywords
Related Articles
- EP ID EP380981
- DOI 10.4274/tjps.80299
- Views 120
- Downloads 0
Study of the Tableting Properties of MCR, a Newly Coprocessed Cellulose-based Direct Compression Excipient
Objectives: In this work, the aim was to coprocess and evaluate a new cellulose-based direct compression tableting excipient (MCR) of improved functionalities by granulation and slugging from locally extracted microcryst...
An In Vitro Study on the Cytotoxicity and Genotoxicity of Silver Sulfide Quantum Dots Coated with Meso-2,3-dimercaptosuccinic Acid
Objectives: Silver sulfide (Ag2S) quantum dots (QDs) are highly promising nanomaterials in bioimaging systems due to their high activities for both imaging and drug/gene delivery. There is insufficient research on the to...
Comprehensive Study on Thiadiazole-Based Anticancer Agents Inducing Cell Cycle Arrest and Apoptosis/Necrosis Through Suppression of Akt Activity in Lung Adenocarcinoma and Glioma Cells
Objectives: Akt is considered as an attractive target for anticancer drug discovery and development and therefore extensive efforts have been devoted to the discovery of new potent anticancer agents targeting Akt. Materi...
Development of Andrographolide-Carboxymethyl Chitosan Nanoparticles: Characterization, in vitro Release and in vivo Antimalarial Activity Study
Objectives: The purpose of this study was to investigate the effect of andrographolide-carboxymethyl chitosan nanoparticles formation on the physical characteristics, in vitro release profile and in vivo antimalarial act...
Intrinsic Stability Study and Forced Degradation Profiling of Olopatadine Hydrochloride by RP-HPLC-DAD-HRMS Method
Objectives: Forced degradation determines the intrinsic stability of a molecule by establishing degradation pathways in order to identify the likely degradation products (DPs). The objective of the present research was t...