Cytotoxic effect of Berberis libanotica roots extracts on human cancer cells and antioxidant activities
Journal Title: UNKNOWN - Year 2017, Vol 6, Issue 6
Abstract
In the present study, we evaluated the cytotoxic effect and the antioxidant activity of methanolic and ethanolic roots extracts obtained from Berberis libanotica a Lebanese medicinal tree. Cytotoxic activity was assessed on the colon cancer HT 29, HCT 116, Caco-2 and breast cancer MCF-7 and MDA-MB-231cell lines, using the MTT viability assay. Both extracts inhibited cancer cells proliferation in a doseand time depending manner without being cytotoxic against the normal MCF-10 cell line. Our results suggest that methanolic extract could induce a caspase-independent cell death in the colon and breast tumor cells HT 29 and MCF-7, respectively. DPPH and FRAP assays showed a moderate to strong antioxidant activity of the methanolic and ethanolic extracts with EC50 values of 0.13 ± 0.001 and 0.1± 0.002 mg/ml, respectively. Collectively, these findings suggest that Berberis libanotica roots could serve as a promising source of antioxidant and anticancer bioactive compounds.
Authors and Affiliations
Kamar Hamade, Louna Karam, Jean Habib, Raghida Abou Merhi, Assem Elkak
Keywords
Related Articles
- EP ID EP451261
- DOI -
- Views 91
- Downloads 0
Purified solasodine from Solanum mauritianum Scop. and its molecular mechanism of antimetastatic potential
Breast cancer has been identified as one of the most common type of cancer among female human population. General treatment approaches against breast cancer includes radio, chemo or hormonal therapy. Unfortunately, most...
Antimicrobial activity of some plant species used for the medical purpose in Turkey
İn this study, the antimicrobial activities of eight species plants, used for treatment of various diseases, were investigated. The extracts of Rhus coriaria L., Pistacia terebinthus L. subsp. palestina, Centaurea virgat...
Pharmacognosy and Phytochemical Evaluation of Hygrophila auriculata (Schumach.) Heine. Root
The genus Hygrophila auriculata (Schumach.) Heine belongs to family Acanthaceae found in India. Commonly known Kokilaksa, as in Sanskrit. Root and seeds used as tonic, for asthama and dysentery (Bhuktar, 2000). Decoction...
Study of Diet and Regimen in the Pathogenesis of Hridroga (Heart Diseases) in Ayurveda
Purpose: Hridroga is the disease of marma (vital organ), which is the seat of many vital activities. Hridroga is among those diseases mentioned in ayurveda where in the descriptions is quite brief and ayurvedic viewpoint...
Anti-tussive activity of Shwasakuthara Rasa a Herbo-mineral formulation prepared with and without Kajjali (Black Sulphide of Mercury) in SO2 induced cough in swiss albino mice
Introduction: Kajjali is considered as the base in maximum Rasa Yogas (Herbo-mineral formulations). Shwasakuthara Rasa (SKR) is a well-known herbo-mineral formulation indicated in different kinds of Shwasa (respiratory d...