Cytotoxic Effect of Hydroalcoholic Extract from Satureja bachtiarica Bunge on Hela Cancer Cells Line
Journal Title: Journal of Herbal Drugs - Year 2017, Vol 7, Issue 4
Abstract
Background & Aim: Cervical cancer is the most common female genital cancer and the second most common cancer in women in the world. Since the herbal ingredients have been used for centuries to treat cancer, purpose of the present study was to determine the cytotoxic effect of hydroalcoholic extract from Satureja bachtiarica Bunge on Hela cancer cells line. Experimental: Hela cells line and natural fibroblast cells line were cultured in RPMI 1640 and DMEM Containing fetal bovine serum and antibiotics, respectively. The cells were exposed to different doses of hydroalcoholic extract of Satureja bachtiarica Bunge (0.156, 0.312, 0.625, 1.25, and 2.5 mg/ml) and were incubated for 24, 48 and 72 hr, respectively. After the incubation period, the modified MTT colorimetric test was used in order to determine cell toxicity of the extract. Results: The results of MTT test Showed that hydroalcoholic extract of Satureja bachtiarica Bunge has dose-dependent anti-cancer effect on Hela cancer cells, so that by increasing the concentration of extract and incubation in 72h, the most percentage of cell death was observed (p < 0.05). The extract of plant didn’t show any significant cytotoxicity on natural fibroblast cell line. Therefore, it seems that its compounds will be applied in treating cancer through more researches. Recommended applications/industries: Considering the low side effects of herbal medicines, especially antioxidants including Satureja bachtiarica Bunge can be used to treat cancer with drug therapy.
Authors and Affiliations
Anahita Behdarvand Shoushtar; Hossein sazegar; Abdollah Ghasemi Pirbaloti
Keywords
Related Articles
- EP ID EP23526
- DOI -
- Views 320
- Downloads 19
Extraction and investigation on the essential oil of Anthemis hyalina DC .in Qazvine province
Background & Aim: This research, order to study of extraction and investigation on the essential oil of Anthemis hyalina DC. were conducted. Material & Methods: The volatile constituents of Anthemis hyalina DC. were...
Preparation and physicochemical analysis of a traditional beverage based on D. Sophia
Background & Aim: The main aim of this study was stabilizing D. Sophia beverage and reduce its sedimentation rate. Experimental: Xanthan and Carboxy methyl cellulose gums have been used in three levels (0.05, .1 and ....
Herbal remedy to relieve of dysmenorrhea by students of Iranshahr Universities in 2010
Background & Aim: Treatment with herbal medicines is very popular in all over the World. Nowadays, using medicinal plants has increased because of undesirable effects of the chemical drugs. More than 10000 plant species...
Effect of oven and shade drying methods on essential oil yield and chemical composition of Kelussia odoratissima Mozaff.
Background & Aim: Kelussiaodoratissima Mozaff is a valuable plant of Apiaceae family that is used as medicinal and aromatic herb. According to the importance of drying treatment on quality and quantity of essential oil,...
Effects of different medium on morphophysiological traits of Lippia citriodora H.B.K. during in vitro culture condition
Background & Aim: Lemon verbena (Lippia citriodora H.B.K.) is a richly scented herb and has a long history as a sacred and medicinal plant. Select an appropriate medium in tissue culture is a necessity for success Experi...