Cytotoxic effect of methanolic extracts of Fritillaria imperialis bulbs and Eryngium caucasicum leaves on hepatoma and colon cancer cells
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2019, Vol 9, Issue 8
Abstract
Objective: To evaluate antitumor activities of Fritillaria imperialis and Eryngium caucasicum methanolic extracts on human hepatoma (HepG2) and colon cancer (HCT116) cell lines in comparison to human foreskin fibroblasts as the normal cells. Methods: Methanolic extracts of Fritillaria imperialis and Eryngium caucasicum were prepared by the maceration method. The effect of the extracts at various concentrations (100, 200, 400, 600, and 800 μg/mL) on cell survival was evaluated using the MTT method. Besides, fluorescence staining was used to evaluate death patterns of the cells. Results: MTT assay showed that Fritillaria imperialis significantly decreased the viability of all cell lines after 24 and 48 hours of treatments. However, Eryngium caucasicum extract did not show any significant cytotoxicity effect on the cell lines. Fluorescence staining revealed that Fritillaria imperialis induced apoptosis of HCT116 cells at 550 μg/mL. Conclusions: Fritillaria imperialis extract has antiproliferative and cytotoxic effects on HCT116 and HepG2 cancer cells and therefore, may serve as an anticancer agent.
Authors and Affiliations
Mostafa Kardan, Zahra Yazdani, Zaher Morsaljahan, Mohammad Ali Ebrahimzadeh, Alireza Rafiei
Keywords
Related Articles
- EP ID EP617254
- DOI 10.4103/2221-1691.262084
- Views 57
- Downloads 0
Antioxidant compounds produced by Pseudocercospora sp. ESL 02, an endophytic fungus isolated from Elaeocarpus sylvestris
Objective: To isolate endophytic fungi from Elaeocarpus sylvestris (E. sylvestris) and to isolate antioxidant compounds from a potential source fungus. Methods: Endophytic fungi were isolated from fresh leaves and stems...
Construction and expression of a synthetic gene encoding nonstructural glycoprotein NS1 of dengue 2 virus in Pichia pastoris
Objectives: To express and characterize NS1 of Indonesian-specific DENV2 virus in Pichia pastoris (P. pastoris). Methods: A codon optimized synthetic gene derived from the DENV-2 NS1 amino acid sequences was synthesized...
Evaluation of berries of Phytolacca dodecandra for growth inhibition of Histoplasma capsulatum var. farciminosum and treatment of cases of epizootic lymphangitis in Ethiopia
Objective To evaluate the berries of Phytolacca dodecandra (P. dodecandra) for its effect on Histoplasma capsulatum var. farciminosum (HCF) and for the treatment of cases of epizootic lymphangitis (EL). Methods Sample...
Candida albicans isolated from urine: Phenotypic and molecular identification, virulence factors and antifungal susceptibility
Objective: To isolate Candida albicans (C. albicans) from the urine of hospitalized patients and assess the virulence factors and antifungal susceptibility profiles of the isolates. Methods: Yeasts were identified using...
Metabolic disturbance and phytochemical changes in Andrographis paniculata and possible action mode of andrographolide
Objective: To explore the effect of gibberellic acid (GA3) and its inhibitor paclobutrazol (PBZ) on chemical composition and their pharmacological effects on Andrographis paniculata (Burm. f.) Wall. ex Nees, and to clari...