Cytotoxic Effect of Thiabendazole on Hn5 Head and Neck Squamous Cell Carcinoma Cell Line

Journal Title: Journal of Dentistry, Shiraz University of Medical Sciences - Year 2017, Vol 18, Issue 3

Abstract

Statement of the Problem: Evidence shows thiabendazole has the potential to inhibit angiogenesis in melanoma and fibrosarcoma; however, its effect on oral squamous cell carcinoma has not been previously studied. Purpose: This study sought to assess the cytotoxic effects of thiabendazole on HN5 head and neck squamous carcinoma cell line. Materials and Method: HN5 cell lines were exposed to different concentrations of thiabendazole (prepared from 99% pure powder) for 24, 48 and 72 hours. Cell viability was assessed by the methyl thiazol tetrazolium assay, and IC50 of thiabendazole was calculated. Cells were also exposed to different concentrations of thiabendazole for 48 hours to determine its effect on expression and transcription of vascular endothelial growth factor gene. Expression of vascular endothelial growth factor mRNA was assessed by real-time polymerase chain reaction. The vascular endothelial growth factor release was assessed by the enzyme-linked immunosorbent assay test. Results: In all concentrations of thiabendazole except for 200 and 550μM, cell viability was significantly different at different time points (p< 0.05). At 48 and 72 hours, cell viability at all concentrations of thiabendazole (100-650μM) significantly decreased compared to the control group (zero concentration). In addition, cell viability significantly decreased with an increase in thiabendazole concentration. At 48 hours, expression of vascular endothelial growth factor mRNA was significantly lower in presence of 500μM thiabendazole compared to the control group (p< 0.001) and release of vascular endothelial growth factor was inhibited in a dose-dependent manner. Conclusion: Thiabendazole inhibited the proliferation of HN5 cells in a dose-dependent and time-dependent manner. It also inhibited the expression of vascular endothelial growth factor gene.

Authors and Affiliations

Amir Jalal Abassi, Abdolreza Mohamadnia, Seyed Alireza Parhiz, Nahid Azizi Moghadam, Naghmeh Bahrami

Keywords

Related Articles

Ergonomic Assessment of Exposure to Musculoskeletal Disorders Risk Factors among Dentists of Shiraz, Iran

Statement of the Problem: In dental profession, exposure to risk factors of musculoskeletal disorders raises the probability of musculoskeletal injuries in different parts of the body. Purpose: This study aimed to deter...

Correlation between Skeletal Age and Metacarpal Bones and Metacarpophalangeal Joints Dimensions

Statement of the Problem: Currently, two major methods have been introduced for bone age assessment using left hand radiography. The first approach is Greulich and Pyle, which is very subjective. The second method is Tan...

Pain Perception Due to Dental Injection by Smartject: Split Mouth Design Study

Statement of the Problem: Dental injection is one of the most fearful procedures in dental setting, especially for children. Many researchers have attempted to find a painless method. As computer controlled local anesthe...

Photogrammetric Comparison of Facial Soft Tissue Profile before and after Protraction Facemask Therapy in Class III Children (6-11 Years Old)

Statement of the Problem: Achieving a normal soft tissue facial profile is considered to be the main concern of class III patients and the goal of most class III treatments. Purpose: The purpose of this study was to inve...

Comparison between Glenoid Fossa Roof Thickness in TMD and non-TMD Patients, a CBCT Study

Statement of the Problem: Various pathologic conditions can affect the thickness of the roof of glenoid fossa (RGF). The relationship between the RGF thickness and temporomandibular joint disorder (TMD) is not explicitly...

Download PDF file
  • EP ID EP240394
  • DOI -
  • Views 113
  • Downloads 0

How To Cite

Amir Jalal Abassi, Abdolreza Mohamadnia, Seyed Alireza Parhiz, Nahid Azizi Moghadam, Naghmeh Bahrami (2017). Cytotoxic Effect of Thiabendazole on Hn5 Head and Neck Squamous Cell Carcinoma Cell Line. Journal of Dentistry, Shiraz University of Medical Sciences, 18(3), 219-226. https://europub.co.uk/articles/-A-240394