Cytotoxic Effect of Thiabendazole on Hn5 Head and Neck Squamous Cell Carcinoma Cell Line
Journal Title: Journal of Dentistry, Shiraz University of Medical Sciences - Year 2017, Vol 18, Issue 3
Abstract
Statement of the Problem: Evidence shows thiabendazole has the potential to inhibit angiogenesis in melanoma and fibrosarcoma; however, its effect on oral squamous cell carcinoma has not been previously studied. Purpose: This study sought to assess the cytotoxic effects of thiabendazole on HN5 head and neck squamous carcinoma cell line. Materials and Method: HN5 cell lines were exposed to different concentrations of thiabendazole (prepared from 99% pure powder) for 24, 48 and 72 hours. Cell viability was assessed by the methyl thiazol tetrazolium assay, and IC50 of thiabendazole was calculated. Cells were also exposed to different concentrations of thiabendazole for 48 hours to determine its effect on expression and transcription of vascular endothelial growth factor gene. Expression of vascular endothelial growth factor mRNA was assessed by real-time polymerase chain reaction. The vascular endothelial growth factor release was assessed by the enzyme-linked immunosorbent assay test. Results: In all concentrations of thiabendazole except for 200 and 550μM, cell viability was significantly different at different time points (p< 0.05). At 48 and 72 hours, cell viability at all concentrations of thiabendazole (100-650μM) significantly decreased compared to the control group (zero concentration). In addition, cell viability significantly decreased with an increase in thiabendazole concentration. At 48 hours, expression of vascular endothelial growth factor mRNA was significantly lower in presence of 500μM thiabendazole compared to the control group (p< 0.001) and release of vascular endothelial growth factor was inhibited in a dose-dependent manner. Conclusion: Thiabendazole inhibited the proliferation of HN5 cells in a dose-dependent and time-dependent manner. It also inhibited the expression of vascular endothelial growth factor gene.
Authors and Affiliations
Amir Jalal Abassi, Abdolreza Mohamadnia, Seyed Alireza Parhiz, Nahid Azizi Moghadam, Naghmeh Bahrami
Keywords
Related Articles
- EP ID EP240394
- DOI -
- Views 104
- Downloads 0
Decompression or Marsupialization; Which Conservative Treatment is Associated with Low Recurrence Rate in Keratocystic Odontogenic Tumors? A Systematic Review
Various treatment modalities have been reported for keratocystic odontogenic tumors (KOT), with different recurrence rates. Marsupialization and decompression are two different conservative surgical techniques for the tr...
Quercetin Decreases Th17 Production by Down-Regulation of MAPK- TLR4 Signaling Pathway on T Cells in Dental Pulpitis
Statement of the Problem: Quercetin is a pharmacological flavonoid that can inhibit high mobility group box1 (HMGB1) protein, a non-histone nuclear protein that is implicated in inflammation. Th17 cells are important cel...
Effective Factors on Oral Health Behaviors of 12-year-old Children in Cities and Villages of Iran: a Path Analysis
Statement of the Problem: Oral and general health status depends on several factors such as the individual’s personal attributes, behaviors, and perceptions. Purpose: This study aimed to evaluate the factors affecting t...
Efficiency of Castor Oil as a Storage Medium for Avulsed Teeth in Maintaining the Viability of Periodontal Ligament Cells
Statement of the Problem: Researchers always seek a new storage medium for avulsed teeth. Castor oil is a vegetable oil with several advantages such as antimicrobial and antioxidant properties, low toxicity, and glutathi...
Shear Bond Strength of a Multi-Mode Adhesive to Bur-Cut and Er,Cr:YSGG Lased Dentin in Different Output Powers
Statement of the Problem: Universal or multi-mode adhesives are new adhesive systems that can be used in both etch-and-rinse (ER) and self-etch (SE) modes. Lesser technical sensitivity and dual use of these adhesives hav...