CYTOTOXICITY, ANTI-POLIOVIRUS ACTIVITY AND IN SILICO BIOLOGICAL EVALUATION OF CONSTITUENTS FROM MAYTENUS GONOCLADA (CELASTRACEAE)

Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 10

Abstract

Objective: The in silico free access web tools PASS online and ChemMapper were used to predict potential biological activities of compounds 1 to 8 isolated from Maytenus gonoclada (Celastraceae). The constituents 4’-O-methylepigalocatequin (6), tingenone (7) and proanthocyanidin A (8), and ethanolic extracts were subjected to in vitro cytotoxicity using VERO cells and anti-Poliovirus assays.Methods: QSAR and molecular superposition, correlating the average number of pharmacophores were used in the prediction studies. Cellular line VERO ATCC CCL-81 was used to determine anti-Poliovirus effect, observed by colorimetric (MTT) method. The annexing V/propidium iodide assay was used to determine the occurrence of apoptosis in the cytotoxicity assays.Results: The experimental results found for constituents 6-8 were in accordance with observed data obtained through PASS online and ChemMapper simulation.Conclusion: Compound 7 showed higher cytotoxic and apoptosis induction properties, and 6 and 8 presented anti-Poliovirus activity.  

Authors and Affiliations

Mauro Lucio G Oliveira, Regina Aparecida Gomes Assenco, Grácia Divina De Fátima Silva, Julio César D Lopes, Fernando César Silva, Maria Célia Da Silva Lanna, José Carlos Magalhães, Lucienir Pains Duarte, Sidney Augusto Vieira Filho

Keywords

Related Articles

A REVIEW ON BIOLOGICAL IMPORTANCE OF PYRIMIDINES IN THE NEW ERA

Pyrimidines occupy an important position in the medicinal world as it has a number of diverse biological properties. Their related fused heterocycles are of interest as potential bioactive molecules. Pyrimidine derivativ...

ANALYSIS OF FATTY ACIDS FROM OIL OF GREEN TEA (CAMELLIA SINENSIS L) BY GAS CHROMATOGRAPHY COUPLED WITH FLAME IONIZATION DETECTOR AND ITS ANTICANCER AND ANTIBACTERIAL ACTIVITY IN VITRO

Objective: Tea is a widely consumed beverage worldwide. The effect of green tea is mainly due to its high polyphenols-(-) epigallocatechin-3-gallate (EGCG) content in the culture of cancer cell and bacterial cells. The p...

EVALUATION OF THE GENOTOXICITY OF EURYCOMA LONGIFOLIA AQUEOUS EXTRACT (PHYSTA®) USING IN VITRO AMES TEST AND IN VIVO MAMMALIAN MICRONUCLEUS TEST

Objective: In this study the genotoxic potential of Eurycoma longifolia aqueous extract (PHYSTA®) was investigated by the in vitro Ames test (Salmonella/microsome mutation assay) and the in vivo mouse peripheral blood c...

SPECTROPHOTOMETRIC DETERMINATION OF PROPRANOLOL HYDROCHLORIDE AND METOPROLOL TARTRATE IN PHARMACEUTICAL DOSAGE FORMS, SPIKED WATER AND BIOLOGICAL FLUIDS

Objective: A new spectrophotometric method for the determination of propranolol hydrochloride (PRO) and metoprolol tartrate (MTP), beta blocker drugs, has been developed for their analysis in pharmaceutical dosage forms...

POTENTIAL DRUG-DRUG INTERACTIONS IN HEART FAILURE PATIENTS

Objective: The aim of the present study was to assess the prevalence, risk rating and the severity of hazardous pDDIs (potential drug-drug interactions) in the prescribed pharmacotherapy in the hospital discharged heart...

Download PDF file
  • EP ID EP579414
  • DOI -
  • Views 71
  • Downloads 0

How To Cite

Mauro Lucio G Oliveira, Regina Aparecida Gomes Assenco, Grácia Divina De Fátima Silva, Julio César D Lopes, Fernando César Silva, Maria Célia Da Silva Lanna, José Carlos Magalhães, Lucienir Pains Duarte, Sidney Augusto Vieira Filho (2014). CYTOTOXICITY, ANTI-POLIOVIRUS ACTIVITY AND IN SILICO BIOLOGICAL EVALUATION OF CONSTITUENTS FROM MAYTENUS GONOCLADA (CELASTRACEAE). International Journal of Pharmacy and Pharmaceutical Sciences, 6(10), 130-137. https://europub.co.uk/articles/-A-579414