Cytotoxicity assessment of the stem bark of Tieghemella heckelii Pierre ex. A Chev. (Sapotaceae) towards Vero and RD human cancer Cell Lines
Journal Title: The Journal of Phytopharmacology - Year 2017, Vol 6, Issue 1
Abstract
The present study aimed at investigating the cytotoxicity of the stem bark of Tieghemella heckelii Pierre ex. A Chev (Sapotaceae). For this purpose, plant extracts were put into contact separately with the different Vero and human RD cancer cell lines diluted in a 96 wells microplate after 24-hour incubation at 37°C. Thereafter, the absorbance was measured every 24 hour for two days, using Elisa reader spectrophotometer. The IC50 values obtained from multi-dose testing of ethanol extract against Vero cell lines ranged from 0.051 to 0.192 mg/mL. The results also showed Vero cell viability of 80.2%, and a mortality rate of 94.9% against RD cell lines whereas methanol extract displayed for the same experiment an IC50 ranging from 0.018 to 2.98 mg/mL with a cell viability of 67%, and a mortality rate of 95.6%. From these results, it could be concluded that the methanol extract of the stem bark showed higher cytotoxic activity towards RD cell lines. As for the ethanol extract, it showed significant non-cytotoxicity towards the Vero cell lines. In the light of this evidence, it can be claimed that the plant exhibited non-cytotoxic patterns against Vero cells and has anticancer potential.
Authors and Affiliations
Bertin Kipré Guédé, Aya Nathalie Guessennd-Kouadio, Jules N’guessan. Kouadio, Mamidou Witabouna Koné
Keywords
Related Articles
- EP ID EP49710
- DOI -
- Views 171
- Downloads 0
Role of Dhoopan Karma and Gauradya Ghrita in the management of Dushta Vrana (infected wound)
Ayurveda is that which deals with good, bad, happy and unhappy life, its promoters and non-promoters, measurement and nature. Shalya Tantra is a specialized branch of Ayurveda in which Surgical, Medicinal and Parasurgi...
Antibacterial properties of the extracts of Allexis obanensis and Allexis batangae (Violaceae) collected at Kribi (South Cameroon)
The present work presents the antibacterial activities of organic and aqueous extracts of Allexis batangae and Allexis obanensis (violaceae). These plants were collected in the locality of Kribi (South Cameroon). The le...
Sedative activity of ethanolic and aqueous extracts of Euphorbia hirta
Insomnia is persistent difficulty in falling asleep that affects regular day time activities. It can induce significant psychological and physical disorder. Insomnia is treated pharmacologically and nonpharmacologically...
Phytochemical screening and antimicrobial activities of the stem bark of Allanblackia parviflora Chev. (Clusiaceae)
This study has successfully explored the photochemistry and antimicrobial properties of the methanolic, aqueous and pet ether stem bark extracts of Allanblackia parviflora (Chevalier) (Clusiaceae). The qualitative phyt...
Resistance modulation studies of vernolide from Vernonia colorata (Drake) on ciprofloxacin, amoxicillin, tetracycline and erythromycin
Resistance modulatory activities of natural products on traditional antibiotics have gained scientific interest in recent years. This is aimed at maximizing antimicrobial potency with major strides in curbing microbial...