Cytotoxicity evaluation of vancomycin and its complex with beta-cyclodextrin on human glial cell line
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2012, Vol 25, Issue 4
Abstract
The possible cytotoxic effects of vancomycin and its complex with beta-cyclodextrin (β-CD) on human glial cell line (CRL 8621) were studied accordingly by means of MTS assay. The cultured cells were incubated with various concentrations of vancomycin, β-CD as well as β-CD/vancomycin complex ranging from 4.69 to 300 ug/ml. A linear dose-dependency cytotoxicity followed by hermetic-like biphasic dose-dependence was observed after incubation period of 72 hours. In general, significant increase (p<0.001) of cell proliferation was observed at lower concentrations: <18.75 μg/ml for cells treated with β-CD and their complex while < 9.38 μg/ml for cells treated with vancomycin. In contrary, regardless of the treatments given, significant (p<0.001) reduce in cell survival was found at higher concentrations >150 μg/ml. In particular, 50 % inhibitory in vitro was achieved at the concentrations of 115.95 μg/ml (for β-CD), 116.48 μg/ml (for vancomycin) and 115.44 μg/ml (for β-CD/vancomycin complex).
Authors and Affiliations
Zarif Sofian, Jafri Abdullah, Afidah Rahim, Shazrin Shafee, Zulkifli Mustafa, Shariza Razak
Keywords
Related Articles
- EP ID EP156138
- DOI -
- Views 80
- Downloads 0
Analysis on diagnostic value of esophageal electrophysiological examination for positive atropine test
To discuss the cause of positive atropine test and the possible reason for false positive atropine test, providing a certain aids for clinical diagnosis and treatment. Eighty patients, conducted atropine test due t...
Formulation development and optimization: Encapsulated system of atenolol and Glyburide
Purpose: Objective of this study is to develop; tablet- in- a capsule system, to deliver Atenolol 25mg and Glyburide 5mg in the hard gelatin capsule. In order to improve patient compliance and reduce problems assoc...
Simultaneous quantitation of Ofloxacin, Fexofenadine HCl and Diclofenac Potassium in affixed dose combinative formulation by HPLC-UV method
A high-pressure liquid chromatography (HPLC-UV) based simple and specific method for simultaneous quantitative determination of Ofloxacin, Fexofenadine HCl and Diclofenac Potassium has been developed and validated...
Analgesic, anti-inflammatory and anti-pyretic activities of Thymus linearis
The present study was aimed to investigate the antipyretic, analgesic and anti-inflammatory activity of aqueous methanolic and n-hexane extract of Thymus linearis. For measuring analgesic activity, writhing test, h...
Marine green algae Codium iyengarii as a good bio-sorbent for elimination of reactive black 5 from aqueous solution
The green seaweeds Codium iyengarii (C. iyengarii) was used to prepare as an adsorbent surface for the deletion of Reactive Black 5 (RB 5) from aqueous solution via adsorption. The batch technique was adopted und...