Cytotoxicity induced by cypermethrin in Human Neuroblastoma Cell Line SH-SY5Y
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2016, Vol 23, Issue 1
Abstract
The purpose of this study was to evaluate the cytotoxic potential of Cypermethrin (CM) on cultured human Neuroblastoma SH-SY5Y cells. SH-SY5Y cells were treated with CM at 0–200µM for 24, 48, and 72 h, [i]in vitro[/i]. It was found that CM induced the cell death of Neuroblastoma cells in a dose- and time-dependent manner, as shown by LDH assays. Next, some aspects of the process of cell death triggered by CM in the human SH-SY5Y cell line were investigated. It was revealed that the pan-caspase inhibitor Q-VD-OPh, sensitizes SH-SY5Y cells to necroptosis caused by CM. Furthermore, signal transduction inhibitors PD98059, SL-327, SB202190, SP600125 failed to attenuate the effect of the pesticide. Finally, it was shown that inhibition of TNF-a by Pomalidomide (PLD) caused statistically significant reduction in CM-induced cytotoxicity. Overall, the data obtained suggest that CM induces neurotoxicity in SH-SY5Y cells by necroptosis.
Authors and Affiliations
Grzegorz Raszewski, Marta Lemieszek, Krzysztof Łukawski
Keywords
Related Articles
- EP ID EP79323
- DOI 10.5604/12321966.1196863
- Views 130
- Downloads 0
Maternal passive smoking during pregnancy and neonatal health.
The objective of the study was the evaluation of effect of passive smoking among pregnant women on the health condition of their newborn babies. The study covered 150 healthy, non-smoking women from the Lublin Region. Am...
Serological survey of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, and Ehrlichia canis infections in rural and urban dogs in Central Italy
[b]Introduction[i][/i][/b][i]. Borrelia burgdorferi [/i]sensu lato (s.l.) and Anaplasma phagocytophilum are well known zoonotic pathogens, whereas[i] Ehrlichia canis[/i] is usually considered to be of veterinary concern,...
Past obstetric history and risk of ovarian cancer
Introduction: Early age at menarche and late age at last menstrual period, as well as other reproductive factors, may be the cause of development of some types of cancer concerning the female reproductive organs. It has...
Oral squamous cell carcinoma (OSCC) – molecular, viral and bacterial concepts
Head and neck cancers constitute the sixth most common malignant tumours worldwide, and are one of the majors problems of global public health. In the oral cavity there are several types of oral cancers, but around 90% a...
Effect of selected socio-demographic, clinical and biochemical factors on self-reported quality of life among post-menopausal women with osteoporosis
Introduction: The goal of health care with relation to women with osteoporosis is the prevention of fractures, maintenance of independence, and good quality of life. Objective: To discover how selected socio-demographic,...