Degenerative Effect of Cisplatin on Testicular Germinal Epithelium
Journal Title: Advanced Pharmaceutical Bulletin - Year 2012, Vol 2, Issue 2
Abstract
Purpose: The present study was designed to explore the effect of intraperitoneal administration of cisplatin in germinal epithelium of mice. There are few reports on the side effect of cisplatin on spermatogenesis when are used as anticancer drug. Therefore, in the present study the effect of cisplatin on spermatogenesis was evaluated by electron microscopy. Methods: Twenty balb/c mice aging 6-8 weeks was used in this study. The mice were divided into two groups, control and cysplatin treated. cysplatin was injected for five days as 2.5 mg /kg. The mice were sacrificed after 5 weeks and testicular specimens were removed, fixed in boueins, formaldeyd fixative and 2.5% Glutaraldehide then prepared for light and electron microscopic study. Results: Observation with optic microscope in treated group thickness of germinal epithelium was reduced a lot and increased the number of apoptotic cells. In some seminiferous tubules only sertoli cells were observed and nucleus of spermatogony cells was hetrochromatin. The electron microscopic observations showed some irregularity waviness and thickening in basal layer. Also myoid cells of this group were thick and contracted. In this group many apoptotic cells and damaged organelles were seen. Conclusion: It was indicated that cisplatin affected testicular germinal epithelium by both cytotoxic effect and induction of apoptosis.
Authors and Affiliations
Daryosh Mohammadnejad, Ali Abedelahi, Jafar Soleimani-rad, Ameneh Mohammadi-roshandeh, Morteza Rashtbar, Ayda Azami
Keywords
Related Articles
- EP ID EP151126
- DOI 10.5681/apb.2012.026
- Views 96
- Downloads 0
Effects of Enzyme Induction and/or Glutathione Depletion on Methimazole-Induced Hepatotoxicity in Mice and the Protective Role of N-Acetylcysteine
Purpose: Methimazole is the most convenient drug used in the management of hyperthyroid patients. However, associated with its clinical use is hepatotoxicity as a life threatening adverse effect. The exact mechanism of m...
Antiproliferative effect of gynura procumbens (lour.) Merr. Leaves etanolic extract on 7,12-dimethylbenz(a)antracene induced male rat liver
Purpose: The leaves of Gynura procumbens (Lour.) Merr. has been traditionally used as anticancer. Ethanolic extract of G. procumbens leaves (EGP) showed cytotoxic activity and anticancer activity in animal cancer model....
Virulence and Antimicrobial Resistance in Enterococci Isolated from Urinary Tract Infections
Purpose: Urinary tract infection (UTI) is the most common nosocomial infection among hospitalized patients. Meanwhile, most frequent infections involving enterococci affect the urinary tract. The aims of this study were...
Hollow microspheres for gastroretentive floating- pulsatile drug delivery: preparation and in vitro evaluation
Purpose: A multiparticular floating-pulsatile drug delivery system was developed for time and site specific drug release of piroxicam. A blend of floating and pulsatile principles of drug delivery system would have the a...
Effect of Taurine on the antimicrobial efficiency of Gentamicin
Context: Gentamicin is mainly used in severe infections caused by gram-negatives. However toxicity including nephrotoxicity and ototoxicity is one of the most important complications of its treatment. The production of f...