DEGRADATION OF FLAVONOIDS BY Cryptococcus albidus α-L-RHAMNOSIDASE
Journal Title: Biotechnologia Acta - Year 2018, Vol 10, Issue 5
Abstract
The aim of the work was to investigate the possibility of practical use substrate specificity of α-Lrhamnosidase Cryptococcus albidus. p-Nitrophenyl derivatives of monosaccharides were used to determine the activity and specificity of the enzyme. The ability to hydrolyze of natural substrates was evaluated by Davis and high-performance liquid chromatography methods. It was shown that the enzyme exhibits narrow specificity towards the glycon of synthetic substrates and hydrolyzes only p-nitrophenyl-α-L-rhamnopyranoside (Km 4.5 mM) and p-nitrophenyl-α-D-glucopyranoside (Km 10.0 mM). C. albidus α-L-rhamnosidase the most active degrades naringin (Km 0.77 mM), releasing prunin and naringenin. Km for neohesperidin was 3.3 mM. The efficacy of the naringin hydrolysis in grapefruit and pomelo juice was 98 and 94% in 60 min (40 оC, 2 U/ml). As the result of treatment by α -L-rhamnosidase of green tea and orange juice, there was a decrease in the content of rutin, narirutin and hesperidin, indicating that the α -1,2- and α -1,6-linked rhamnose can be cleaved from natural flavonoids. Thus, the study shows the efficiency of treating citrus juices and green tea with C. albidus α -L-rhamnosidase for the purpose of improving their taste qualities and obtaining bioavailable flavonoids glucosides.
Authors and Affiliations
N. V. Borzova, O. V. Gudzenko, L. D. Varbanets
Keywords
Related Articles
- EP ID EP423779
- DOI 10.15407/biotech11.05.035
- Views 172
- Downloads 0
BIOTECHNICAL INFORMATION SYSTEMS FOR MONITORING OF CHEMICALS IN ENVIRONMENT: BIOPHYSICAL APPROACH
The newest biotechnical systems for environment ecological monitoring based on the use of modern information and computer technologies and existing databases of chemical substances have been analyzed. In particular, ther...
MICROBIAL SYNTHESIS OF PHYTOHORMONES
The aim of the review was to analyze current literature data and the results of own studies on the synthesis of auxins, cytokinins, and gibberellins by plant-associated microorganisms (living in rhizosphere, endophy...
Lactobacillus AS PRODUCERS OF EXTRACELLULAR TANNASE
The aim of the work was to find strains of lactic acid bacteria capable to synthesize extracellular tannase enzyme − the key enzymehe hydrolyzing tannins which are plant food constituent. One of the main product of tann...
Biological databases using object-oriented system analysis
The aim of the work was to develop electronic database of biological organisms (fishes, viruses) on the base of the methods that have been used before in mathematics, engineering, physics. The methods of object-oriented...
ELECTRONIC INFORMATION SYSTEMS FOR MONITORING OF POPULATIONS AND MIGRATIONS OF INSECTS
The purpose of the work was to analyze existing information systems (IS) of biological objects and to propose the methods for development of such IS for insects on the example of Noctuidae (Lepidoptera). A detailed analy...