DEGRADATION OF FLAVONOIDS BY Cryptococcus albidus α-L-RHAMNOSIDASE
Journal Title: Biotechnologia Acta - Year 2018, Vol 10, Issue 5
Abstract
The aim of the work was to investigate the possibility of practical use substrate specificity of α-Lrhamnosidase Cryptococcus albidus. p-Nitrophenyl derivatives of monosaccharides were used to determine the activity and specificity of the enzyme. The ability to hydrolyze of natural substrates was evaluated by Davis and high-performance liquid chromatography methods. It was shown that the enzyme exhibits narrow specificity towards the glycon of synthetic substrates and hydrolyzes only p-nitrophenyl-α-L-rhamnopyranoside (Km 4.5 mM) and p-nitrophenyl-α-D-glucopyranoside (Km 10.0 mM). C. albidus α-L-rhamnosidase the most active degrades naringin (Km 0.77 mM), releasing prunin and naringenin. Km for neohesperidin was 3.3 mM. The efficacy of the naringin hydrolysis in grapefruit and pomelo juice was 98 and 94% in 60 min (40 оC, 2 U/ml). As the result of treatment by α -L-rhamnosidase of green tea and orange juice, there was a decrease in the content of rutin, narirutin and hesperidin, indicating that the α -1,2- and α -1,6-linked rhamnose can be cleaved from natural flavonoids. Thus, the study shows the efficiency of treating citrus juices and green tea with C. albidus α -L-rhamnosidase for the purpose of improving their taste qualities and obtaining bioavailable flavonoids glucosides.
Authors and Affiliations
N. V. Borzova, O. V. Gudzenko, L. D. Varbanets
Keywords
Related Articles
- EP ID EP423779
- DOI 10.15407/biotech11.05.035
- Views 151
- Downloads 0
ELECTRONIC INFORMATION SYSTEMS FOR MONITORING OF POPULATIONS AND MIGRATIONS OF INSECTS
The purpose of the work was to analyze existing information systems (IS) of biological objects and to propose the methods for development of such IS for insects on the example of Noctuidae (Lepidoptera). A detailed analy...
SOME TRENDS IN MATHEMATICAL MODELING FOR BIOTECHNOLOGY
The purpose of present research is to demonstrate some trends of development of modeling methods for biotechnology according to contemporary achievements in science and technique. At the beginning the general approaches...
DEGRADATION OF FLAVONOIDS BY Cryptococcus albidus α-L-RHAMNOSIDASE
The aim of the work was to investigate the possibility of practical use substrate specificity of α-Lrhamnosidase Cryptococcus albidus. p-Nitrophenyl derivatives of monosaccharides were used to determine the activity and...
CALIX[4]ARENES METHYLENE BISPHOSPHONIC ACIDS EFFECT ON PLASMIN ACTIVITY
The aim of the work was to study plasmin inhibiting properties of calix[4]arenes functionalized by phosphonic acid remnants. The following methods were used: turbidimetry, analysis of enzyme activity for chromogenic subs...
ANTIAMYLOIDOGENIC EFFECT OF MiR-101 IN EXPERIMENTAL ALZHEIMER’S DISEASE
The aim of the study was to determine the effect of miR-101 on the level of β-amyloid peptide and activation of the cytokine system in the brain regions of animals with an experimental model of Alzheimer’s disease. MiR-1...