Dentinal Tubules of Attrited, Abraded and Eroded Teeth- A Comparative Evaluation of Scanning Electron and Light Microscope
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2016, Vol 16, Issue 2
Abstract
Aim: The aim was to study the ultrastructure of dentin exposed by attrition, abrasion and erosion separately and in combination and to visualize the lesions using scanning electron microscope and thereby establish a superior basis for evaluating dentinal changes at the ultra-structural level and to correlate the findings with changes seen at the light microscopic level. Study Design: Tooth wear is often multifactorial. Loss and excessive wear of hard dental tissues is a permanent problem of the dentition, especially in present era, encompassing almost all age groups. In this study 40 extracted teeth were used. Four groups were made and comparison was made between light microscope and scanning electron microscopic findings. Place and Duration of Study: Department of oral and maxillofacial pathology, career post graduate institute of dental sciences, lucknow, (U.P.), India. Methodology: Forty extracted permanent teeth (10 attrited, 10 abraded, 10 eroded and 10 normal premolars and molars) were sampled divided into four groups. After debridement and fixation in 10% formalin for 24-48 hours, the teeth were fractured along their longitudinal axes. Two halves of the teeth were studied under scanning electron and light microscope. The dentinal changes secondary to attrition, abrasion and erosion such as dead tracts, dentinal sclerosis and reparative dentin formation seen under light microscopy, were correlated with the ultra-structural findings. The data so obtained were tabulated and statistically analyzed. Results: The light microscopic finding of 4 randomly selected teeth, showed frequency of tertiary dentin significantly more than dead tracts and dentinal sclerosis was absent, from each group. 10 samples scanned with electron microscope showed the tubular surface/margins smooth, tubular surface/margins rough and presence of crystals was highly significant (p<0.001) whereas peritubular dentin and intertubular dentin, was not significant (p>0.05) in each group. Similarly, mean density of dentinal tubules of normal group was the highest followed by abraded, attrited and eroded the least. The percentage of affected dentinal tubules was most in eroded & least in attrited teeth. Conclusion: The scanning electron microscope is a powerful magnification tool which offers extremely high resolution. In the present study, scanning electron microscopic image complements the information available from the light microscope about the dentinal changes in abraded, attrited and eroded teeth.
Authors and Affiliations
Mudita Chaturvedi, Balasundari Shreedhar, Mala Kamboj, Saurabh Chaturvedi
Keywords
Related Articles
- EP ID EP341192
- DOI 10.9734/BJMMR/2016/25812
- Views 26
- Downloads 0
Prevalence of Hashimoto’s Thyroiditis and Its Association with Vitamin D Deficiency in West Bengal, India
Objective: The aim of our study is to screen the prevalence of Hashimoto’s Thyroiditis and to evaluate a correlation between 25(OH)Vitamin D deficiency and AITD in hypothyroid patients of West Bengal. Study Design: Ethic...
Pulse-induced Continuous Cardiac Output Monitor versus Trans-esophageal Doppler Monitor for Optimization of Fluid Management in Patients Undergoing Major Abdominal Surgery. A Comparative Study
Background: Perioperative fluid management is essential to the practice of anaesthesia. Outcomes may be improved if fluid therapy is individualized according to the patient's fluid responsiveness. Pulse-induced cont...
Women Attending Hospital in Malawi for Miscarriage: A Qualitative Study of Perceptions of Miscarriage
Aims: To investigate women’s perceptions of miscarriage and its complications. Study Design: A qualitative design was used with data collected through semi-structured interviews. Place and Duration of Study: Gynaecology...
Factors Associated with Successful Smoking Cessation in Tunisian Smokers: Findings from the Smoking Cessation Clinic in the University Hospital of Sahloul, Sousse, Tunisia
Aims: To identify the determinants of successful smoking cessation among the attendees of the smoking cessation clinic of the University Hospital of Sahloul. Place and Duration of the Study: The smoking cessation clinic...
Colon Obstruction and Gradual Regression of Giant Pseudopolyps in Inflammatory Bowel Disease
Inflammatory pseudopolyps associated with inflammatory bowel disease occur on inflamed colon mucosa due to the regeneration and healing of ulcerated epithelium. They are classified as giant when they are over 1.5-cm in d...