DESCRIPTION OF IN VITRO CULTURES FOR SOME SPONTANEOUS LIGNICOLOUS BASIDIOMYCETES SPECIES
Journal Title: Analele Stiintifice ale Universitatii Alexandru Ioan Cuza din Iasi, Serie noua, Sectiunea II a. Biologie vegetala - Year 2012, Vol 58, Issue 2
Abstract
13 species of lignicolous basidiomycetes from 7 taxonomic families and 4 orders, Class Agaricomycetes, Phyllum Basidiomycota, have been studied. The mycelium grown in vitro was analysed and the culture description of these isolates was presented. The dikaryotic mycelium from the context of the fruit bodies was used as source of inoculum in the isolation processes. Petri dishes of 9 cm diameter filled with malt extract-agar media (malt extract 2 %) were used for the culturing purpose. After the inoculation, the plates were incubated at 25 °C, in the dark, for 6 weeks. Observations made directly and using a Nikon stereomicroscope were employed in order to measure the growth rhythm and to assess the changes of the mycelium: edge of colony, surface, reverse, shape, colour, smell, presence or absence of the exudates. Microscopic slides were made after 6 weeks from the inoculation for investigations concerning the types of hyphae, the colour and the structure of the mycelium. Particular elements, such as: cuticular cells, chlamydospores, arthroconidia, conidia, basidia and crystals were also noticed. We observed similar characters for our isolates but also significant differences between them. The growth rhythm varied strongly, the slowest growth rhythm being recorded for isolates of Ganoderma applanatum and Xylobolus frustulatus.
Authors and Affiliations
TIBERIUS BALAEŞ, CĂTĂLIN TĂNASE
Keywords
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