DESCRIPTION OF IN VITRO CULTURES FOR SOME SPONTANEOUS LIGNICOLOUS BASIDIOMYCETES SPECIES
Journal Title: Analele Stiintifice ale Universitatii Alexandru Ioan Cuza din Iasi, Serie noua, Sectiunea II a. Biologie vegetala - Year 2012, Vol 58, Issue 2
Abstract
13 species of lignicolous basidiomycetes from 7 taxonomic families and 4 orders, Class Agaricomycetes, Phyllum Basidiomycota, have been studied. The mycelium grown in vitro was analysed and the culture description of these isolates was presented. The dikaryotic mycelium from the context of the fruit bodies was used as source of inoculum in the isolation processes. Petri dishes of 9 cm diameter filled with malt extract-agar media (malt extract 2 %) were used for the culturing purpose. After the inoculation, the plates were incubated at 25 °C, in the dark, for 6 weeks. Observations made directly and using a Nikon stereomicroscope were employed in order to measure the growth rhythm and to assess the changes of the mycelium: edge of colony, surface, reverse, shape, colour, smell, presence or absence of the exudates. Microscopic slides were made after 6 weeks from the inoculation for investigations concerning the types of hyphae, the colour and the structure of the mycelium. Particular elements, such as: cuticular cells, chlamydospores, arthroconidia, conidia, basidia and crystals were also noticed. We observed similar characters for our isolates but also significant differences between them. The growth rhythm varied strongly, the slowest growth rhythm being recorded for isolates of Ganoderma applanatum and Xylobolus frustulatus.
Authors and Affiliations
TIBERIUS BALAEŞ, CĂTĂLIN TĂNASE
Keywords
Related Articles
- EP ID EP98505
- DOI -
- Views 155
- Downloads 0
FOLIAR RESPONSE REACTIONS INDUCED BY ATMOSPHERIC POLLUTANTS ON THE AESCULUS HIPPOCASTANUM L. AND TILIA TOMENTOSA L. SPECIES FROM IAŞI CITY AREA
In this paper we present the foliar response reaction induced by atmospheric pollutants on the Aesculus hippocastanum L. and Tilia tomentosa L. species situated around Iaşi city`s air quality monitoring stations. Pollut...
HYPHOLOMA MYOSOTIS (FR.) M. LANGE (BASIDIOMYCOTA, FUNGI) THE FIRST RECORD IN ROMANIA
Hypholoma myosotis (Fr.) M. Lange is a saprophytic species rarely spread in the oligotrophic peat bogs on the territory of Europe and North America. The species is being signaled for the first time on the territory...
CHARACTERIZATION OF MORPHOLOGY AND GERMINATION CAPACITY IN VIOLA ODORATA L. POLLEN
The paper presents some general morphological aspects of Viola odorata L. pollen, with diagnosis value for this taxon. We have also shown the germination ability of sweet violet pollen, according to glucide concent...
USE AND POSSIBLE SIGNIFICANCE OF TWO SPECIES OF BORAGINACEAE FAMILY IN PREHISTORY – A REVIEW OF THE CUCUTENI CULTURE FINDS
Our paper aims at presenting and discussing the discovery of Lithospermum officinale L. and Buglossoides purpurocaerulea (L.) I. M. Johnston seeds deposits from archaeological contexts. The nutlets deposits that we discu...
CONTRIBUTION TO THE KNOWLEDGE OF WEEDS VEGETATION ALONG THE TISA EVERGLADE
In this paper, two vegetal associations with a nonnative character (Helianthetum tuberosi (Moor 1958) Oberd. 1967 and Reynoutrietum japonicae Görs 1974 corr. Hilbig 1995) are presented. These associations (less kno...