DESIGN AND DEVELOPMENT OF NOVEL MICROEMULSION BASED TOPICAL FORMULATION OF HESPERIDIN
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 12
Abstract
Objective: Bioflavonoid hesperidin is used primarily to assist treatment of capillary disorders like hemorrhoids and varicose veins, by reducing capillary permeability. There are certain limitations to the use of these bioflavonoid in the pharmaceutical formulations because of their physical properties like limited aqueous solubility, poor bioavailability and high oral dose. Therefore in the present research work micro emulsions of hesperidin were developed for improving solubility and bioavailability using topical route.Methods: Micro emulsions of hesperidine were prepared by plotting pseudo ternary phase diagram using eucalyptus oil, Tween 80, AccononCC6 and water. The micro emulsion with optimized droplet size, polydispersity index and ex vivo diffusion ability was then converted into the ointment for ease of application. Droplet size and Polydispersity index were obtained by using Photon correlation spectroscopy. Optimized o/w microemulsion of hesperidin composed of eucalyptus oil 20%, Tween-80/Acconon CC-6(2:1) 33% and water was then converted into ointment using optimized ointment base. [a1] The microemulsion loaded ointment was then characterized for physicochemical parameters. Ex-vivo permeation and In-vivo bioavailability studies were carried out to check the r. release profile of drug from the prepared formulations.Results: It was observed from ex-vivo permeation studies that flux value for optimized microemulsion was found to be 8.971µg/ml/cm2as compared to pure hesperidin (1.230 µg/ml/cm2). This formulation was then converted into ointment by using optimized base containing PEG-6000, PEG-400 and Cetyl alcohol. This microemulsion based ointment passed all the characterization tests (droplet size analysis using PCS, ex-vivo permeation studies, in-vivo bioavailability studies etc.) as well as remained stable for the period of 3 mo during stability studies as per ICH guidelines. The bioavailability studies of hesperidin microemulsion based ointment in rats showed 3 fold statistically significant (p<0.001) improvement in bioavailability as compared to microemulsion when applied topically.Conclusion: Thus it can be concluded that components of microemulsion and ointment are contributing to improve bioavailability of hesperidin.Â
Authors and Affiliations
Vaishali Kilor, Nidhi Sapkal, Gunjan Vaidya
Keywords
Related Articles
- EP ID EP578047
- DOI -
- Views 78
- Downloads 0
ANTIMICROBIAL ACTIVITY AND POTENCY OF CASSIA ABBREVIATA OLIV STEM BARK EXTRACTS
Objective: To evaluate the potential antimicrobial activity and relative potency of aqueous and organic extracts of Cassia abbreviata Oliv stem bark against clinical isolates of Neisseria gonorrhoeae (NG), Pseudomonas ae...
ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF SIX ALGERIAN MEDICINAL PLANTS
Objective: This work had two objectives: the first, to evaluate the total phenolic or flavonoid contents of six Algerian medicinal plants and second, to determine whether these compounds have antioxidant properties and a...
DEVELOPMENT AND VALIDATION OF NEW RP-HPLC METHOD FOR THE ESTIMATION OF LINEZOLID IN LINEZOLID GEL
Objective: Development and validation of new RP-HPLC method for the estimation of linezolid in linezolid gel.Methods: Linezolid was chromatographed on a reverse phase symmetry C18 column (150 x 4.6 mm x 3.5 µm) in a mob...
SCREENING OF PATHOGENIC AEROMONAS SPECIES FROM MARKETED FISH SAMPLES
Aeromonas spp. have received increasing attention as opportunistic as well as primary pathogens in humans, aquatic and terrestrial animals. Aeromonads are common contaminants in foods such as fish and other sea foods, ra...
THE EFFECTS OF DIABETES MELLITUS ON THE RESPONSE TO PEGINTERFERON-ALPHA IN COMBINATION WITH RIBAVIRIN THERAPY IN EGYPTIAN CHRONIC HEPATITIS C PATIENTS
Objective: The present study aimed to determine whether type 2 diabetes mellitus influences the response to antiviral therapy with peg-interferon alpha plus ribavirin in Egyptian patients with chronic hepatitis C.Methods...