DESIGN AND DEVELOPMENT OF NOVEL MICROEMULSION BASED TOPICAL FORMULATION OF HESPERIDIN
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 12
Abstract
Objective: Bioflavonoid hesperidin is used primarily to assist treatment of capillary disorders like hemorrhoids and varicose veins, by reducing capillary permeability. There are certain limitations to the use of these bioflavonoid in the pharmaceutical formulations because of their physical properties like limited aqueous solubility, poor bioavailability and high oral dose. Therefore in the present research work micro emulsions of hesperidin were developed for improving solubility and bioavailability using topical route.Methods: Micro emulsions of hesperidine were prepared by plotting pseudo ternary phase diagram using eucalyptus oil, Tween 80, AccononCC6 and water. The micro emulsion with optimized droplet size, polydispersity index and ex vivo diffusion ability was then converted into the ointment for ease of application. Droplet size and Polydispersity index were obtained by using Photon correlation spectroscopy. Optimized o/w microemulsion of hesperidin composed of eucalyptus oil 20%, Tween-80/Acconon CC-6(2:1) 33% and water was then converted into ointment using optimized ointment base. [a1] The microemulsion loaded ointment was then characterized for physicochemical parameters. Ex-vivo permeation and In-vivo bioavailability studies were carried out to check the r. release profile of drug from the prepared formulations.Results: It was observed from ex-vivo permeation studies that flux value for optimized microemulsion was found to be 8.971µg/ml/cm2as compared to pure hesperidin (1.230 µg/ml/cm2). This formulation was then converted into ointment by using optimized base containing PEG-6000, PEG-400 and Cetyl alcohol. This microemulsion based ointment passed all the characterization tests (droplet size analysis using PCS, ex-vivo permeation studies, in-vivo bioavailability studies etc.) as well as remained stable for the period of 3 mo during stability studies as per ICH guidelines. The bioavailability studies of hesperidin microemulsion based ointment in rats showed 3 fold statistically significant (p<0.001) improvement in bioavailability as compared to microemulsion when applied topically.Conclusion: Thus it can be concluded that components of microemulsion and ointment are contributing to improve bioavailability of hesperidin.Â
Authors and Affiliations
Vaishali Kilor, Nidhi Sapkal, Gunjan Vaidya
Keywords
Related Articles
- EP ID EP578047
- DOI -
- Views 75
- Downloads 0
COMPARATIVE STUDY OF ANTI-INFLAMMATORY ACTIVITY OF AQUEOUS AND METHANOLIC EXTRACTS OF HIBISCUS CANNABINUS LEAF (MALVACEAE)
Objective: The present study was undertaken to investigate and compare the anti-inflammatory activity of an aqueous and methanolic extract of Hibiscus cannabinus (Malvaceae) using carrageenan-induced rat paw edema.Method...
DESIGN AND IN VITRO CHARACTERIZATION OF MUCOADHESIVE BUCCAL PATCHES OF DULOXETINE HYDROCHLORIDE
Objective: Buccal mucosa is a potential site for the delivery of drugs to the systemic circulation, a drug administered through the buccal mucosa enters directly to the systemic circulation, thereby which bypass the drug...
RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION AND STABILITY INDICATING STUDY OF METFORMIN AND LINAGLIPTIN IN PURE AND PHARMACEUTICAL DOSAGE FORMS
Objective: The objective of this study was to develop a simple, efficient, specific, precise and accurate Reverse phase High Performance liquid chromatography method for the simultaneous estimation of Metformin and Linag...
ANTI-INFECTIVE COATING OF GENTAMICIN SULPHATE ENCAPSULATED PEG/PVA/CHITOSAN FOR PREVENTION OF BIOFILM FORMATION
Objective: The main aim of the current study is to show Anti-infective coating on biomedical implants, one of the novel approaches to control the biofilm formation.Methods: In this study, the coating was employed by the...
DEVELOPMENT OF OLMESARTAN MEDOXOMIL-LOADED CHITOSAN MICROPARTICLES: A POTENTIAL STRATEGY TO IMPROVE PHYSICOCHEMICAL AND MICROMERITIC PROPERTIES
Objective: The objective of the present research was to improve physicochemical and micromeritic properties of Olmesartan Medoxomil (OLM), BCS class II antihypertensive drug by loading in Chitosan (CH) microparticles.Met...