Design and simulate routine serologic training experiments in blood transfusion using RhD blood group

Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 7

Abstract

Objective To design and simulate routine serological experiments in transfusion techniques using RhD blood group, so as to solve the problem of difficult to obtain positive specimens in experimental teaching. Methods RhD positive red blood cells, RhD negative red blood cells and anti-D reagent were used to design and simulate the enzyme treatment experiment, absorption and elution test, antibody identification experiment and cross matching experiment of polybrene technology in transfusion techniques. Results Papain treatment of red blood cells made the agglutination of RhD positive red blood cells and IgG anti-D visible. Absorption and elution test were successfully simulated with RhD positive red blood cells and IgG anti-D reagent. The antibody identification of anti-Jka and anti-Fya was successfully simulated by creating different identification panel and panel cells made by RhD positive cells and negative cells. Cross matching test of polybrene method can also be simulated using RhD negative and positive red blood cells and IgG anti-D reagent. Conclusion RhD blood group can be used to simulate most of the routine serological experiments of blood transfusion, which can be used in the serological laboratory teaching of transfusion.

Authors and Affiliations

Yingying NIU, Jiang WANG, Chunxia CHEN, Chunyan HUANG

Keywords

Related Articles

Effect of adding tranexamic acid during surgery on blood loss and security during short segment lumbar spinal stenosis surgery

Objective To discuss the effect of adding tranexamic acid(TXA) during surgery on blood loss and security during short segment lumbar spinal stenosis surgery. Methods One hundred and eight patients with lumbar spinal sten...

Research progress of neonatal low-threshold platelet transfusion strategy

Neonatal thrombocytopenia is one of the common hematological abnormalities in neonates, especially premature infants. Platelet transfusion is an effective method of treating neonatal thrombocytopenia, but its threshold s...

Explore of nanopore sequencing technology in ambiguities of HLA genotyping

[Objective] To resolve the ambiguities of HLA genotyping generated by next generation sequencing (NGS) using nanopore sequencing technology. [Methods] A total of 38 samples with ambiguous HLA genotyping by NGS in our lab...

Application of anti-idiotypic antibodies in antibody screening and crossmatch tests of patients treated with CD47 monoclonal antibody

Objective To perform pre-transfusion examination and major crossmatch test using CD47 anti-idiotypic antibody (CD47 AID) (method 1) and reagent lack of anti-IgG4 anti-human globulin(method 2) in patients treated with CD4...

Blood group serology and gene detection in patients with B(A) subgroup in Jiaozhou, Qingdao

Objective To investigate and analyze the serological and molecular biological characteristics of B(A) subgroup in a tertiary hospital in Jiaozhou, Qingdao. Methods From November 2019 to February 2023, the samples of 12 p...

Download PDF file
  • EP ID EP738602
  • DOI 10.13303/j.cjbt.issn.1004-549x.2023.07.023
  • Views 53
  • Downloads 0

How To Cite

Yingying NIU, Jiang WANG, Chunxia CHEN, Chunyan HUANG (2023). Design and simulate routine serologic training experiments in blood transfusion using RhD blood group. Chinese Journal of Blood Transfusion, 36(7), -. https://europub.co.uk/articles/-A-738602