“DESIGNING OF STS MARKERS FOR SEVERAL MYCOBACTERIUM SPECIES BY USING AMPLIFIED 16S RIBOSOMAL DNA
Journal Title: INTERNATIONAL JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH - Year 2015, Vol 3, Issue 1
Abstract
In order to diagnose several MOTT species such as M. smegmatis and M. kansasii along with M. tuberculosis, STS markers were designed for 16S rRNA gene conserved region. Designed primer sets were found to be specific for their target 16S regions of particular Mycobacterium species when tested in PCR. Using PCR methodology we have developed precise, sensitive Diagnosis methodology for MOTT species detection by obtaining variable banding patterns in gel electrophoresis. Along with that we have developed fast and inexpensive Mycobacterium genomic DNA isolation protocol which will reduce the cost of diagnosis.
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