Detecting enteropathogenic Campylobacters in chicken feces by PCR and comparing with culture method
Journal Title: مجله دانشگاه علوم پزشکی شهرکرد - Year 2003, Vol 5, Issue 3
Abstract
At present Campylobacters are the main cause of the bacterial diarrhea worldwide. In human beings the disease is transmitted by consumption of contaminated water and foodstuff, especially poultry products. Diagnosis of this bacterium is difficult by the bacteriological tests due to the long incubation period and unavailability of suitable differential tests. Hence, other diagnostic procedures, other than culture, are very demanding. Rectal swabs of 116 chickens from the poultry farms of Isfahan were enriched for 12 to 18 hours in Campy-Thio medium, and cultured in the Campylobacter selective agar medium. Gram staining, oxidase, catalase and hypurate hydrolysis tests and evaluation of sensitivity to nalidixic acid and cephalothin were employed for diagnosis and typing of the samples. For molecular evaluation of the samples, after DNA extraction, PCR was performed using two sets of type specific primers for C.coli and C.jejuni. Using culture, 11 samples (9.5%) were found positive from which 8 were jejuni and 3 were coli. Using PCR, 27 samples (23.2%) were positive, from which 18 were jejuni and 9 were coli. Sensitivity and specificity of PCR procedure in comparison to the culture were 100 and 84.7 percent, respectively. According to the results, all the culture positive samples were diagnosed and genotyped correctly using the PCR procedure. Therefore in view of its considerable sensitivity and speed, PCR could be a very reliable substitute for the culture method in Campylobacter detection in poultry samples.
Authors and Affiliations
mohammad bokaiyan, rasoul salehi, shahram shahraki, mazhar ghorayshi
Keywords
Related Articles
- EP ID EP85825
- DOI -
- Views 75
- Downloads 0
The comparison of ultrasonic and orbscan corneal pachymetry in corneal thickness measurement in the patients referred to Aban eye clinic, Isfahan
Background and aim: With increased popularity of laser surgeries as a treatment for refractory errors and importance of pre-operative pachymetry for decreasing the complications of refractive surgery, correct measurement...
بررسی عوامل خطر زای انفارکتوس میوکارد در بیماران مراجعه کننده به بیمارستانهای استان چهارمحال و بختیاری در سال 1384
زمينه و هدف: شناسایی عوامل خطرزای انفارکتوس میوکارد مهمترين اقدام در جهت به حداقل رساندن، تعديل و اصلاح عوامل خطرزا در جمعيت هاي در معرض خطر است که نتیجه آن کاهش عوارض، کاهش مرگ و میر و در نهایت حفظ نیروی مولد جامعه است....
کارآئی فن آوری اکسيداسيون پيشرفته ازن و هيدروکسيل در تجزيه متيل – ترشياری - بوتيل اتر (MTBE
زمينه و هدف: فرآيندهای اکسيداسيون پيشرفته بعنوان روش های سريع و موثر برای تصفيه دامنه وسيعی از آلايندهای آلی مقاوم به اثبات رسيده است. هدف از اين تحقيق آزمايش استفاده از O3 در ترکيب با يونهای هيدروکسيل (pH بالا) بعنوان ت...
Comparison of histopathologic findings of non-tumoral gastric mucus of patients with gastric cancer and patients with chronic gastritis
Background and aim: Gastric cancer is the second cause of cancer death throughout the world. Identification of precancerous lesions is important for designing preventive measures. Therefore, the aim of this study was to...
The effect of self-care programe on foot healing in diabetic patients
Background and aim: Diabetes is a major endocrine disorder and is characterized by metabolic abnormalities, retinopathy, neuropathy, nephropathy and atherosclerosis. In this disease, the patients play important role in...