Detection and Assay of Antimycobacterial AgentIsoniazid Utilizing Isocratic High PerformanceLiquid Chromatography
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 5
Abstract
Isoniazid was assayed by HPLC, utilizing a reversed-phase C-18 column with eluent solvent (5.3% ethanol, 93.7% water, 1% acetic acid). Samples for analysis were prepared in distilled water as the solvent. Detection of isoniazid was accomplished at 265 nm, which showed highest sensitivity for isoniazid . Column pump pressure approximately 2100 psig, rise time 0.1 with flow rate 1.0 mL/minute. Elution of isoniazid occurred at 2.2 minutes. Limit of detection (LOD) of isoniazid was 1.172 x 10-5 molar, but with detection going as high as 1.460 x 10-3 molar which provides a detection range of 125x. The limit of quantitation (LOQ) is 3.905 x 10-5 molar. Reverse phase isocratic conditions is shown to be effective for determination of isoniazid in aqueous based samples. The standard curve is highly linear reaching as high as 1.460 x 10-3 molar (y = 50570789.86 x), having coefficient of determination (R2 = 0.9916) with very strong positive correlation coefficient (r= 0.9958). The percent recovery of drug ranged from 96% to 104%. Utilizing reversed phase column with isocratic solvent conditions (ethanol, acetic acid, and water in column solvent), is effective for determination of isoniazid.
Authors and Affiliations
Ronald Bartzatt 1 ,, Purnima Gajmer1, Mai Han Cassandra Nguyen1 ,, Alexandra My-Hanh Tran1
Keywords
Related Articles
- EP ID EP390943
- DOI 10.9790/3008-1205024047
- Views 87
- Downloads 0
Effect of Aqueous Extract of Defatted Flaxseeds (Linum Usitatissimum Linn) on Fructose-Induced Hypertension in Rats by Inhibiting Angiotensin-Converting Enzyme (ACE)
Linum usitatissimum Linn. is a widely acclaimed medicinal plant used in every household. The seeds of L. usitatissimum have both medicinal and nutraceutical properties.The study was conducted to evaluate the anti-hyperte...
Immunomodulatory Potential of Gemmomodified Extract of Terminalia arjuna
.In recent years there is increasing interest in the field ofmodulation of immune by plant products. The aim of this study was to evaluate the immunomodulatory potential of bark and gemmomodified extracts of Terminalia a...
Low Hemoglobin Levels in Infected Diabetic Foot Ulcer
Background: Studies have shown that anemia is twice as common in diabetics compared with nondiabetics. Objective: We aimed to evaluate the prevalence of anemia in diabetic foot ulceration (DFU) patients and it’s correlat...
Isolation and Identification of Multi-Drug Resistant Acinetobacter baumannii from a tertiary health care centre of Bangladesh
Acinetobacter baumannii has emerged over the last decade as a significant opportunistic pathogen and primarily associated with hospital-acquired infections. Increasing multidrug resistance pattern of A. baumannii makes i...
Faunal Diversity of Ajmer Aravalis Lepidoptera Moths
Ajmer is located in the center of Rajasthan (INDIA) between 25 0 38 “ and 26 0 58 “ North 75 0 22” East longitude covering a geographical area of about 8481sq .km hemmed in all sides by Aravalli hills . About 7 miles fro...