DETECTION AND PURIFICATION OF HARMFUL COMPOUNDS IN BEVERAGES USING HPLC
Journal Title: JOURNAL OF ADVANCES IN CHEMISTRY - Year 2016, Vol 12, Issue 20
Abstract
The presence of harmful compounds like caffeine and carbonated compounds in different beverages like soft drinks, fruit juices deserves great attention because of its toxic and carcinogenic effects on human beings. We report on the detection and purification of those substances with the help of HPLC(High Performance Liquid Chromatography).According to the migration rate, stationary phase and mobile phase, retention time we can extract the desire compounds. Depending upon the solvent and sample we can detect the compounds with the help of the detector.The chromatogram will be displayed and it can be viewed in the PC with the help of Osiris software. Compounds like Caffeine, Aspartame, Neotame, Saccharin, Maltodextrin, sucrose, fructose etc can be detected and purified. Detection and purification takes place in the column of HPLC where the process called adsorption takes place. Retention time can be calculated by the total time taken of a component that spends in both mobile phase and stationary phase. It is always expressed in minutes
Authors and Affiliations
Rukkumani V, Saravanakumar M
Keywords
Related Articles
- EP ID EP652989
- DOI 10.24297/jac.v12i20.4749
- Views 193
- Downloads 0
Synthesis and physico-chemical studies of a novel coordination compound ZnCl2(C6H4FNH2)2
A new Zn(II) complex with the monodentate ligand 3-fluoroaniline, ZnCl2(C6H4FNH2)2, has been prepared and characterized by single crystal X-ray diffraction, Solid state NMR, IR and UV-visible spectroscopies. The Zn(II) i...
Computational Study of the Stability of Tautomers and equilibrium constants of Cyanuric acid (CA) in Different solvents.
In the present investigation, the tautomeric and equilibrium of Cyanuric acid has been studied using Hartifock (HF) method in the gas phase and different solvents using the PCM model. The relative energies of these tauto...
PROPERTIES FOR NONLINEAR SOIL STRUCTURE INTERACTION ANALYSIS FOR SHALLOW FOUNDATION
The formulation of a soil structure interaction problem begins with identifying the underlying design variables which are primarily varied during the analysis process. In design, construction and maintenance of any Engin...
IMPACT OF PRESSURE ON THE PERFORMANCE OF PROTON EXCHANGE MEMBRANE FUEL CELL
The Proton Exchange Membrane (PEM) fuel cell performance not only depends on temperature but also depends on the operating pressure, which will increase the performance of the PEM fuel cell. The PEM fuel cell with serpen...
Extraction-Spectrophotometric Studies on the Complex Formation of Iron(III) with 4-(2-Thiazolylazo)Resorcinol and Tetrazolium Salts
Four liquid-liquid extraction-chromogenic systems containing Fe(III), 4-(2-thiazolylazo)resorcinol (TAR), tetrazolium salt (TZS), water and chloroform were studied. 2,3,5-Triphenyl-2H-tetrazolium chloride (TTC), 3-(4,5-d...